| Literature DB >> 25240884 |
Murugan Subramanian1, Xiao Ling Li, Toshifumi Hara, Ashish Lal.
Abstract
We have recently developed a biochemical approach to isolate miRNA-bound mRNAs and have used this method to identify the genome-wide mRNAs regulated by the tumor suppressor miRNA miR-34a. This method involves transfection of cells with biotinylated miRNA mimics, streptavidin pulldown, RNA isolation, and qRT-PCR. The protocol in this chapter describes these steps and the issues that should be considered while designing such pulldown experiments.Entities:
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Year: 2015 PMID： 25240884 PMCID： PMC6378880 DOI： 10.1007/978-1-4939-1369-5_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745