Philippe Vasseur1, Iris Devaure2, Jacques Sellier3, Adriana Delwail4, Carine Chagneau-Derrode2, Florian Charier2, David Tougeron5, Jean-Pierre Tasu3, Hanitriniaina Rabeony4, Jean-Claude Lecron6, Christine Silvain5. 1. Department of Hepato-Gastroenterology, Poitiers University Hospital, 86021 Poitiers, France; Laboratoire Inflammation Tissus Epithéliaux et Cytokines EA 4331, Pôle Biologie Santé, 86022 Poitiers, France. Electronic address: philippe.vasseur@chu-poitiers.fr. 2. Department of Hepato-Gastroenterology, Poitiers University Hospital, 86021 Poitiers, France. 3. Department of Radiology, Poitiers University Hospital, 86000 Poitiers, France. 4. Laboratoire Inflammation Tissus Epithéliaux et Cytokines EA 4331, Pôle Biologie Santé, 86022 Poitiers, France. 5. Department of Hepato-Gastroenterology, Poitiers University Hospital, 86021 Poitiers, France; Laboratoire Inflammation Tissus Epithéliaux et Cytokines EA 4331, Pôle Biologie Santé, 86022 Poitiers, France. 6. Laboratoire Inflammation Tissus Epithéliaux et Cytokines EA 4331, Pôle Biologie Santé, 86022 Poitiers, France; Department of Immunology/Inflammation, Poitiers University Hospital, 86021 Poitiers, France.
Abstract
BACKGROUND/ OBJECTIVES: Pancreatic acinar cells are major targets of IL-22. Our aim is to study early plasma levels of IL-22, of pro- and anti-inflammatory cytokines in acute pancreatitis, and their association with severity or necrosis infection. METHODS: Consecutive patients admitted to the Department of Hepato-Gastroenterology at Poitiers University of Medicine Hospital (France) with a diagnosis of AP were prospectively enrolled. Plasma concentrations of IL-22, IL-6, IL-8, IL-1 α, IL-1β, TNF- α, IFN-γ, IL-17A, IL-10, IL-1ra and IL-4 were assessed by multiple immunoassay at the admission time. A thoracoabdominal contrast-enhanced CT scan was performed at day 2. RESULTS: Sixty-two patients were included; 13 patients (21%) had a severe acute pancreatitis, 5 patients (8%) developed necrosis infection and 29 patients (47%) had pleural effusion. Plasma levels of IL-22 were high in AP (135 ± 31 vs 4.2 ± 1.8 pg/ml for controls, p < 0.05), but did not correlate with the severity of the disease, whereas IL-6, IL-10 and IL-1ra where enhanced in patients with severe acute pancreatitis and with pleural effusion. Patients who further developed necrosis infection had higher levels of IL-1ra at admission (p = 0.0004). CONCLUSION: In acute pancreatitis, high plasma levels of IL-22 are observed, regardless the severity of the disease. In contrast, severe forms were associated with increased levels of IL-6, IL-10 and IL-1ra. The beneficial or deleterious role of IL-22 in AP remains to be further studied.
BACKGROUND/ OBJECTIVES: Pancreatic acinar cells are major targets of IL-22. Our aim is to study early plasma levels of IL-22, of pro- and anti-inflammatory cytokines in acute pancreatitis, and their association with severity or necrosis infection. METHODS: Consecutive patients admitted to the Department of Hepato-Gastroenterology at Poitiers University of Medicine Hospital (France) with a diagnosis of AP were prospectively enrolled. Plasma concentrations of IL-22, IL-6, IL-8, IL-1 α, IL-1β, TNF- α, IFN-γ, IL-17A, IL-10, IL-1ra and IL-4 were assessed by multiple immunoassay at the admission time. A thoracoabdominal contrast-enhanced CT scan was performed at day 2. RESULTS: Sixty-two patients were included; 13 patients (21%) had a severe acute pancreatitis, 5 patients (8%) developed necrosis infection and 29 patients (47%) had pleural effusion. Plasma levels of IL-22 were high in AP (135 ± 31 vs 4.2 ± 1.8 pg/ml for controls, p < 0.05), but did not correlate with the severity of the disease, whereas IL-6, IL-10 and IL-1ra where enhanced in patients with severe acute pancreatitis and with pleural effusion. Patients who further developed necrosis infection had higher levels of IL-1ra at admission (p = 0.0004). CONCLUSION: In acute pancreatitis, high plasma levels of IL-22 are observed, regardless the severity of the disease. In contrast, severe forms were associated with increased levels of IL-6, IL-10 and IL-1ra. The beneficial or deleterious role of IL-22 in AP remains to be further studied.
Authors: Li Jinfeng; Wang Yunliang; Liu Xinshan; Wang Yutong; Wang Shanshan; Xue Peng; Yang Xiaopeng; Xu Zhixiu; Lu Qingshan; Yin Honglei; Cao Xia; Wang Hongwei; Cao Bingzhen Journal: Biomed Res Int Date: 2016-05-31 Impact factor: 3.411