Elizabeth D E Papathanassoglou1, Meropi D A Mpouzika2, Margarita Giannakopoulou3, Evangelos Bozas3, Nicos Middleton4, Sofia Boti5, Andreas Karabinis6. 1. Department of Nursing, Cyprus University of Technology, Limassol, Cyprus e.papathanassoglou@cut.ac.cy. 2. Department of Nursing B, Faculty of Health and Caring Professions, Technological Educational Institute of Athens, Egaleo, Greece. 3. University of Athens, School of Nursing, Athens, Hellas, Greece. 4. Department of Nursing, Cyprus University of Technology, Limassol, Cyprus. 5. Department of Pathophysiology, University of Athens, School of Medicine, Athens, Greece. 6. Surgical Care Unit, Onassis Cardiac Surgery Center, Athens, Greece.
Abstract
INTRODUCTION: In critical illness, apoptotic loss of immunocytes is associated with immunosuppression. AIM: To explore expression of Fas/Fas ligand (FasL) on B and T cells from critically ill patients without sepsis compared to matched controls and associations with disease severity and neuropeptide Y (NPY), cortisol, adrenocorticotropic hormone (ACTH), and prolactin (PRL) levels. METHODS: Repeated-measures correlational design with 36 critically ill patients (14-day follow-up) and 36 controls. Disease severity was assessed using the Multiple Organ Dysfunction Score (MODS) and Multi Organ Failure scale. Fas/FasL values were standardized for viable cell counts. An enzyme-linked immunosorbent assay (NPY) and electrochemiluminescence immunoassay (cortisol, ACTH, and PRL) were employed. RESULTS: Fas and FasL expression on T-helper (p < .0001-.03) and T-cytotoxic cells (p < .0001-.002) and Fas expression on B cells (p < .0001-.03) were higher in patients. MODS severity was associated with FasL expression on cytotoxic T cells (r = .752-.902, p = .023-.037). There was an inverse association between Day 1 NPY levels and Fas expression on T-helper cells (r = -.447, p = .019). On the day of maximum severity, we report for the first time an inverse association between NPY levels and FasL expression on helper (r = -.733, p = .016) and cytotoxic (r = -.862, p = .003) T cells. Cortisol levels were positively associated with counts of FasL-positive helper (r = .828) and cytotoxic (r = .544, p < .05) T cells. CONCLUSION: Results suggest a potential role for stress neuropeptides in lymphocyte survival and activation in critical illness.
INTRODUCTION: In critical illness, apoptotic loss of immunocytes is associated with immunosuppression. AIM: To explore expression of Fas/Fas ligand (FasL) on B and T cells from critically illpatients without sepsis compared to matched controls and associations with disease severity and neuropeptide Y (NPY), cortisol, adrenocorticotropic hormone (ACTH), and prolactin (PRL) levels. METHODS: Repeated-measures correlational design with 36 critically illpatients (14-day follow-up) and 36 controls. Disease severity was assessed using the Multiple Organ Dysfunction Score (MODS) and Multi Organ Failure scale. Fas/FasL values were standardized for viable cell counts. An enzyme-linked immunosorbent assay (NPY) and electrochemiluminescence immunoassay (cortisol, ACTH, and PRL) were employed. RESULTS: Fas and FasL expression on T-helper (p < .0001-.03) and T-cytotoxic cells (p < .0001-.002) and Fas expression on B cells (p < .0001-.03) were higher in patients. MODS severity was associated with FasL expression on cytotoxic T cells (r = .752-.902, p = .023-.037). There was an inverse association between Day 1 NPY levels and Fas expression on T-helper cells (r = -.447, p = .019). On the day of maximum severity, we report for the first time an inverse association between NPY levels and FasL expression on helper (r = -.733, p = .016) and cytotoxic (r = -.862, p = .003) T cells. Cortisol levels were positively associated with counts of FasL-positive helper (r = .828) and cytotoxic (r = .544, p < .05) T cells. CONCLUSION: Results suggest a potential role for stress neuropeptides in lymphocyte survival and activation in critical illness.
Authors: Maria Kapritsou; Dimitrios P Korkolis; Margarita Giannakopoulou; Theodoros Katsoulas; Maria Bastaki; Evangelos A Konstantinou Journal: Asia Pac J Oncol Nurs Date: 2019-07-16