Delphine Girlich1, Laurent Dortet1, Laurent Poirel2, Patrice Nordmann3. 1. INSERM U914 'Emerging Resistance to Antibiotics', Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France. 2. INSERM U914 'Emerging Resistance to Antibiotics', Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland. 3. INSERM U914 'Emerging Resistance to Antibiotics', Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland patrice.nordmann@unifr.ch.
Abstract
OBJECTIVES: To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate. METHODS: The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing. RESULTS: Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR structure, which itself was inserted into a new variant of the Proteus genomic island 1 (PGI1). This new PGI1-PmPEL variant of 64.4 kb was chromosomally located, as an external circular form in the P. mirabilis isolate, suggesting potential mobility. CONCLUSIONS: This is the first known description of the bla(NDM-1) gene in a genomic island structure, which might further enhance the spread of the bla(NDM-1) carbapenemase gene among enteric pathogens.
OBJECTIVES: To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate. METHODS: The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing. RESULTS: Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR structure, which itself was inserted into a new variant of the Proteus genomic island 1 (PGI1). This new PGI1-PmPEL variant of 64.4 kb was chromosomally located, as an external circular form in the P. mirabilis isolate, suggesting potential mobility. CONCLUSIONS: This is the first known description of the bla(NDM-1) gene in a genomic island structure, which might further enhance the spread of the bla(NDM-1) carbapenemase gene among enteric pathogens.
Authors: Alexander M Wailan; Hanna E Sidjabat; Wan Keat Yam; Nabil-Fareed Alikhan; Nicola K Petty; Anna L Sartor; Deborah A Williamson; Brian M Forde; Mark A Schembri; Scott A Beatson; David L Paterson; Timothy R Walsh; Sally R Partridge Journal: Antimicrob Agents Chemother Date: 2016-06-20 Impact factor: 5.191