Literature DB >> 2523516

A DNA probe from Schistosoma mansoni allows rapid determination of the sex of larval parasites.

T K Walker1, D Rollinson, A J Simpson.   

Abstract

A DNA clone representing a 0.4 kb degenerative repeat has been isolated. The DNA sequence is present only in the genome of female Schistosoma mansoni at different stages of the life cycle, at a frequency of approximately 75 copies per adult female genome. The sequence is not expressed and probably represents satellite DNA in the heterochromatin region of the W chromosome. It is demonstrated that the DNA clone may be used for the rapid determination of the sex of cercariae without the need for DNA isolation or Southern blotting.

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Year:  1989        PMID: 2523516     DOI: 10.1016/0166-6851(89)90046-7

Source DB:  PubMed          Journal:  Mol Biochem Parasitol        ISSN: 0166-6851            Impact factor:   1.759


  3 in total

Review 1.  The molecular epidemiology of parasites.

Authors:  G Hide; A Tait
Journal:  Experientia       Date:  1991-02-15

2.  Multiplex PCR for sexing Schistosoma japonicum cercariae and its utility.

Authors:  Jing Xu; Chunxiang Li; Zhongliang Duan; Dan Yu; Tingting Zhang; Huihui Ma; Xiaoli Wang; Tingzheng Zhan; Chaoming Xia
Journal:  Parasitol Res       Date:  2019-08-26       Impact factor: 2.289

Review 3.  DNA probes and PCR for diagnosis of parasitic infections.

Authors:  J B Weiss
Journal:  Clin Microbiol Rev       Date:  1995-01       Impact factor: 26.132

  3 in total

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