Yan Xu1, Biao Liu2, Qun-Li Shi2, Pei-Lin Huang3, Xiao-Jun Zhou2, Heng-Hui Ma2, Zhen-Feng Lu2, Yu Bo2, Staffan Eriksson4, Ellen He5, Sven Skog5. 1. Department of Pathology, Jinling Hospital, Nanjing University School of Medicine Nanjing, P.R.China ; Department of Internal Medicine, Zhong Da Hospital Affiliated to Southeast University Nanjing, P.R.China. 2. Department of Pathology, Jinling Hospital, Nanjing University School of Medicine Nanjing, P.R.China. 3. Department of Internal Medicine, Zhong Da Hospital Affiliated to Southeast University Nanjing, P.R.China. 4. Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences Uppsala, Sweden. 5. Sino-Swed Molecular Bio-Medicine Research Institute Shenzhen, China.
Abstract
OBJECTIVES: The sensitivity and reliability of the biomarkers thymidine kinase 1 (TK1) and Ki-67 were studied in relation to clinical features and prognosis of survival for pathological-T1 (pT1) lung adenocarcinoma patients. METHODS: TK1 and Ki-67 expression was determined in 80 patients with pT1 adenocarcinoma of the lung and in 20 specimens from normal lung tissues, using immunohistochemistry. RESULTS: TK1 was found in most lung tumor cells both in the cytoplasm and the nuclei. The positive labelling index (LI) for total TK1 was significantly higher than that for Ki-67. There was a significant correlation between the LI of total TK1 and lymph node metastasis, degree of tumor invasion and pathologic stages, which was not found for Ki-67. In addition, the overall 5-year survival of patients was statistically significant different between low and high levels of TK1 expression, but not in cases of Ki-67. A multivariate analysis revealed that expression of TK1, lymph node involvement and TNM pathology staging could serve as independent prognostic factors for the disease progression of pT1 lung adenocarcinoma patients. CONCLUSIONS: Compared with Ki-67, TK1 is a more reliable proliferation index in pT1 adenocarcinoma of lung, which can evaluate the invasion and the prognosis of tumor.
OBJECTIVES: The sensitivity and reliability of the biomarkers thymidine kinase 1 (TK1) and Ki-67 were studied in relation to clinical features and prognosis of survival for pathological-T1 (pT1) lung adenocarcinomapatients. METHODS:TK1 and Ki-67 expression was determined in 80 patients with pT1 adenocarcinoma of the lung and in 20 specimens from normal lung tissues, using immunohistochemistry. RESULTS:TK1 was found in most lung tumor cells both in the cytoplasm and the nuclei. The positive labelling index (LI) for total TK1 was significantly higher than that for Ki-67. There was a significant correlation between the LI of total TK1 and lymph node metastasis, degree of tumor invasion and pathologic stages, which was not found for Ki-67. In addition, the overall 5-year survival of patients was statistically significant different between low and high levels of TK1 expression, but not in cases of Ki-67. A multivariate analysis revealed that expression of TK1, lymph node involvement and TNM pathology staging could serve as independent prognostic factors for the disease progression of pT1 lung adenocarcinomapatients. CONCLUSIONS: Compared with Ki-67, TK1 is a more reliable proliferation index in pT1 adenocarcinoma of lung, which can evaluate the invasion and the prognosis of tumor.
Authors: Nicola Ciancio; Maria Grazia Galasso; Raffaele Campisi; Laura Bivona; Marcello Migliore; Giuseppe U Di Maria Journal: Multidiscip Respir Med Date: 2012-09-14