Xuanxuan Zhou1, Lan Ge2, Qian Yang1, Yanhua Xie1, Jiyuan Sun1, Wei Cao1, Siwang Wang1. 1. Department of Natural Medicine, School of Pharmacy, Fourth Military Medical University 169 West Changle Road, Xi'an 710032, China ; The Cultivation Project of Collaborative Innovation Center for Chinese Medicine in QinBa Mountains Xi'an 710032, China. 2. Department of Dermatology, Southwest Hospital, Third Military Medical University Chongqing 400038, China.
Abstract
OBJECTIVE: To research and reveal Tetrahydroxystilbene glucoside (TSG) anti-thinning effect of dermas on skin of ageing mice. METHODS: The dermal layer thickness was measured with hematoxylin-eosin (HE) staining; the levels of collagen and elastic fibers were measured with immunohistochemical staining; the levels of insulin, insulin-like growth factor-1 (IGF-1), and two receptors of insulin and IGF-1 were measured with Elisa kits; the levels of Ca(2+) and P were measured with ELISA kits. RESULTS: TSG and Polygonum multiflorum extract (PME) made thicken dermal layer thickness (P<0.01, vs. control group); promoted collagen fiber expression (P<0.01, vs. control group, 22.94% and 28.26% vs. 20.41%); reduced the levels of insulin (P<0.01, vs. control group, 2.50 ng/ml and 2.69 ng/ml vs. 3.04 ng/ml), insulin like growth factor 1 (IGF-1, P<0.01, vs. control group, 154.75 ng/ml and 155.60 ng/ml vs. 209.28 ng/ml), receptors of insulin (P<0.01, vs. control group, 0.423 ng/ml and 0.426 ng/ml vs. 0.648 ng/ml) and IGF-1 (P<0.01, vs. control group, 71.96 ng/ml and 81.68 ng/ml vs. 87.02 ng/ml) in aging mice skin; raised the levels of Ca(2+) and P in serum in mice (P<0.01, vs. control group, 1.24 mol/ml and 1.30 mol/ml vs. 1.08 mol/ml; P<0.01, vs. control group, 2.00 mol/ml and 2.03 mol/ml vs. 1.197 mol/ml). CONCLUSION: TSG and PME showed their protections to skin aging in mice challenged with control groups. It ensured the anti-thinning effect of dermas of TSG and provided two potential factors (insulin/IGF-1 signal pathway and the level of Ca(2+)) related to skin senescence of aging mice.
OBJECTIVE: To research and reveal Tetrahydroxystilbene glucoside (TSG) anti-thinning effect of dermas on skin of ageing mice. METHODS: The dermal layer thickness was measured with hematoxylin-eosin (HE) staining; the levels of collagen and elastic fibers were measured with immunohistochemical staining; the levels of insulin, insulin-like growth factor-1 (IGF-1), and two receptors of insulin and IGF-1 were measured with Elisa kits; the levels of Ca(2+) and P were measured with ELISA kits. RESULTS:TSG and Polygonum multiflorum extract (PME) made thicken dermal layer thickness (P<0.01, vs. control group); promoted collagen fiber expression (P<0.01, vs. control group, 22.94% and 28.26% vs. 20.41%); reduced the levels of insulin (P<0.01, vs. control group, 2.50 ng/ml and 2.69 ng/ml vs. 3.04 ng/ml), insulin like growth factor 1 (IGF-1, P<0.01, vs. control group, 154.75 ng/ml and 155.60 ng/ml vs. 209.28 ng/ml), receptors of insulin (P<0.01, vs. control group, 0.423 ng/ml and 0.426 ng/ml vs. 0.648 ng/ml) and IGF-1 (P<0.01, vs. control group, 71.96 ng/ml and 81.68 ng/ml vs. 87.02 ng/ml) in aging mice skin; raised the levels of Ca(2+) and P in serum in mice (P<0.01, vs. control group, 1.24 mol/ml and 1.30 mol/ml vs. 1.08 mol/ml; P<0.01, vs. control group, 2.00 mol/ml and 2.03 mol/ml vs. 1.197 mol/ml). CONCLUSION:TSG and PME showed their protections to skin aging in mice challenged with control groups. It ensured the anti-thinning effect of dermas of TSG and provided two potential factors (insulin/IGF-1 signal pathway and the level of Ca(2+)) related to skin senescence of aging mice.
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