Y Yang1, M Hu, Y Zhang, H Li, Z Miao. 1. Department of Medical Genetics, School of Basic Medical Sciences, Peking University, Beijing, 100191, China.
Abstract
OBJECTIVES: Human umbilical cord mesenchymal stem cells (hUCMSCs) play a critical role in expanding haematopoietic stem cells (HSCs) by providing the essential microenvironment for haematopoiesis. In this study, we sought to investigate whether CD29 of hUCMSCs would play a key role in the ability of hUCMSCs to help expand HSCs in vivo and in vitro. MATERIAL AND METHODS: To investigate whether CD29 of hUCMSCs would play a key role for the ability of hUCMSCs to expand HSCs, soluble anti-CD29 antibody was added to co-cultures of hUCMSCs and cord blood (CB) CD34(+) cells. It significantly blocked expansion of CB CD34(+) cells induced by hUCMSCs. Using CD29-deficient hUCMSCs models, long-term culture-initiating cell and non-obese diabetic/severe combined immunodeficient disease mouse repopulating cell assay, revealed that CB CD34(+) cells co-cultured with CD29-deficient hUCMSCs only retained the capacity of multipotent differentiation for 5 weeks at the most. RESULTS: Soluble anti-CD29 antibody significantly blocked expansion of CB CD34(+) cells induced by hUCMSCs. CB CD34(+) cells co-cultured with CD29-deficient hUCMSCs only retained the capacity of multipotent differentiation for 5 weeks at the most. CONCLUSIONS: CB CD34(+) cells co-cultured with CD29-deficient hUCMSCs gave rise to all major haematopoietic lineages, but failed to engraft long term.
OBJECTIVES:Human umbilical cord mesenchymal stem cells (hUCMSCs) play a critical role in expanding haematopoietic stem cells (HSCs) by providing the essential microenvironment for haematopoiesis. In this study, we sought to investigate whether CD29 of hUCMSCs would play a key role in the ability of hUCMSCs to help expand HSCs in vivo and in vitro. MATERIAL AND METHODS: To investigate whether CD29 of hUCMSCs would play a key role for the ability of hUCMSCs to expand HSCs, soluble anti-CD29 antibody was added to co-cultures of hUCMSCs and cord blood (CB) CD34(+) cells. It significantly blocked expansion of CB CD34(+) cells induced by hUCMSCs. Using CD29-deficient hUCMSCs models, long-term culture-initiating cell and non-obese diabetic/severe combined immunodeficient diseasemouse repopulating cell assay, revealed that CB CD34(+) cells co-cultured with CD29-deficient hUCMSCs only retained the capacity of multipotent differentiation for 5 weeks at the most. RESULTS: Soluble anti-CD29 antibody significantly blocked expansion of CB CD34(+) cells induced by hUCMSCs. CB CD34(+) cells co-cultured with CD29-deficient hUCMSCs only retained the capacity of multipotent differentiation for 5 weeks at the most. CONCLUSIONS: CB CD34(+) cells co-cultured with CD29-deficient hUCMSCs gave rise to all major haematopoietic lineages, but failed to engraft long term.
Authors: Tiki Bakhshi; Ryan C Zabriskie; Shamanique Bodie; Shannon Kidd; Susan Ramin; Laura A Paganessi; Stephanie A Gregory; Henry C Fung; Kent W Christopherson Journal: Transfusion Date: 2008-09-16 Impact factor: 3.157