Literature DB >> 25227312

eMethylsorb: electrochemical quantification of DNA methylation at CpG resolution using DNA-gold affinity interactions.

Abu Ali Ibn Sina1, Sidney Howell, Laura G Carrascosa, Sakandar Rauf, Muhammad J A Shiddiky, Matt Trau.   

Abstract

We report a simple electrochemical method referred to as "eMethylsorb" for the detection of DNA methylation. The method relies on the base dependent affinity interaction of DNA with gold. The methylation status of DNA is quantified by monitoring the electrochemical current as a function of the relative adsorption level of bisulphite treated DNA samples onto a bare gold electrode. This method can successfully distinguish methylated and unmethylated epigenotypes at single CpG resolution.

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Year:  2014        PMID： 25227312     DOI： 10.1039/c4cc06732k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  6 in total

1.  Application of bioconjugation chemistry on biosensor fabrication for detection of TAR-DNA binding protein 43.

Authors:  Yifan Dai; Chunlai Wang; Liang-Yuan Chiu; Kevin Abbasi; Blanton S Tolbert; Geneviève Sauvé; Yun Yen; Chung-Chiun Liu
Journal:  Biosens Bioelectron       Date:  2018-06-01       Impact factor: 10.618

2.  An Interfacial Affinity Interaction-Based Method for Detecting HOTAIR lncRNA in Cancer Plasma Samples.

Authors:  Kimberley Clack; Narshone Soda; Surasak Kasetsirikul; Richard Kline; Carlos Salomon; Muhammad J A Shiddiky
Journal:  Biosensors (Basel)       Date:  2022-04-28

3.  Highly sensitive and multiplexed analysis of CpG methylation at single-base resolution with ligation-based exponential amplification.

Authors:  Fengxia Su; Limei Wang; Yueying Sun; Chenghui Liu; Xinrui Duan; Zhengping Li
Journal:  Chem Sci       Date:  2014-12-11       Impact factor: 9.825

4.  Epigenetically reprogrammed methylation landscape drives the DNA self-assembly and serves as a universal cancer biomarker.

Authors:  Abu Ali Ibn Sina; Laura G Carrascosa; Ziyu Liang; Yadveer S Grewal; Andri Wardiana; Muhammad J A Shiddiky; Robert A Gardiner; Hemamali Samaratunga; Maher K Gandhi; Rodney J Scott; Darren Korbie; Matt Trau
Journal:  Nat Commun       Date:  2018-12-04       Impact factor: 14.919

5.  Electrochemically detecting DNA methylation in the EN1 gene promoter: implications for understanding ageing and disease.

Authors:  Amy E Morgan; Katie D Acutt; Mark T Mc Auley
Journal:  Biosci Rep       Date:  2020-11-27       Impact factor: 3.840

6.  Advanced fabrication of biosensor on detection of Glypican-1 using S-Acetylmercaptosuccinic anhydride (SAMSA) modification of antibody.

Authors:  Yifan Dai; Kevin Abbasi; Michael DePietro; Samantha Butler; Chung Chiun Liu
Journal:  Sci Rep       Date:  2018-09-10       Impact factor: 4.379
  6 in total

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