Veiko Vengerfeldt1, Katerina Špilka2, Mare Saag1, Jens-Konrad Preem3, Kristjan Oopkaup3, Jaak Truu3, Reet Mändar4. 1. Department of Stomatology, University of Tartu, Tartu, Estonia. 2. Department of Microbiology, Faculty of Medicine, University of Tartu, Tartu, Estonia. 3. Department of Geography, Faculty of Science and Technology, University of Tartu, Tartu, Estonia; Competence Centre on Reproductive Medicine and Biology, Tartu, Estonia. 4. Department of Microbiology, Faculty of Medicine, University of Tartu, Tartu, Estonia; Competence Centre on Reproductive Medicine and Biology, Tartu, Estonia. Electronic address: reet.mandar@ut.ee.
Abstract
INTRODUCTION: Chronic apical periodontitis (CAP) is a frequent condition that has a considerable effect on a patient's quality of life. We aimed to reveal root canal microbial communities in antibiotic-naive patients by applying Illumina sequencing (Illumina Inc, San Diego, CA). METHODS: Samples were collected under strict aseptic conditions from 12 teeth (5 with primary CAP, 3 with secondary CAP, and 4 with a periapical abscess [PA]) and characterized by profiling the microbial community on the basis of the V6 hypervariable region of the 16S ribosomal RNA gene by using Illumina HiSeq2000 sequencing combinatorial sequence-tagged polymerase chain reaction products. RESULTS: Root canal specimens displayed highly polymicrobial communities in all 3 patient groups. One sample contained 5-8 (mean = 6.5) phyla of bacteria. The most numerous were Firmicutes and Bacteroidetes, but Actinobacteria, Fusobacteria, Proteobacteria, Spirochaetes, Tenericutes, and Synergistetes were also present in most of the patients. One sample contained 30-70 different operational taxonomic units; the mean (± standard deviation) was lower in the primary CAP group (36 ± 4) than in the PA (45 ± 4) and secondary CAP (43 ± 13) groups (P < .05). The communities were individually different, but anaerobic bacteria predominated as the rule. Enterococcus faecalis was found only in patients with secondary CAP. One PA sample displayed a significantly high proportion (47%) of Proteobacteria, mainly at the expense of Janthinobacterium lividum. CONCLUSIONS: This study provided an in-depth characterization of the microbiota of periapical tissues, revealing highly polymicrobial communities and minor differences between the study groups. A full understanding of the etiology of periodontal disease will only be possible through further in-depth systems-level analyses of the host-microbiome interaction.
INTRODUCTION:Chronic apical periodontitis (CAP) is a frequent condition that has a considerable effect on a patient's quality of life. We aimed to reveal root canal microbial communities in antibiotic-naive patients by applying Illumina sequencing (Illumina Inc, San Diego, CA). METHODS: Samples were collected under strict aseptic conditions from 12 teeth (5 with primary CAP, 3 with secondary CAP, and 4 with a periapical abscess [PA]) and characterized by profiling the microbial community on the basis of the V6 hypervariable region of the 16S ribosomal RNA gene by using Illumina HiSeq2000 sequencing combinatorial sequence-tagged polymerase chain reaction products. RESULTS: Root canal specimens displayed highly polymicrobial communities in all 3 patient groups. One sample contained 5-8 (mean = 6.5) phyla of bacteria. The most numerous were Firmicutes and Bacteroidetes, but Actinobacteria, Fusobacteria, Proteobacteria, Spirochaetes, Tenericutes, and Synergistetes were also present in most of the patients. One sample contained 30-70 different operational taxonomic units; the mean (± standard deviation) was lower in the primary CAP group (36 ± 4) than in the PA (45 ± 4) and secondary CAP (43 ± 13) groups (P < .05). The communities were individually different, but anaerobic bacteria predominated as the rule. Enterococcus faecalis was found only in patients with secondary CAP. One PA sample displayed a significantly high proportion (47%) of Proteobacteria, mainly at the expense of Janthinobacterium lividum. CONCLUSIONS: This study provided an in-depth characterization of the microbiota of periapical tissues, revealing highly polymicrobial communities and minor differences between the study groups. A full understanding of the etiology of periodontal disease will only be possible through further in-depth systems-level analyses of the host-microbiome interaction.
Authors: R R D S Oliveira; D Fermiano; M Feres; L C Figueiredo; F R F Teles; G M S Soares; M Faveri Journal: J Dent Res Date: 2016-03-02 Impact factor: 6.116
Authors: Ilona F Persoon; Mark J Buijs; Ahmet R Özok; Wim Crielaard; Bastiaan P Krom; Egija Zaura; Bernd W Brandt Journal: Clin Oral Investig Date: 2016-10-22 Impact factor: 3.573
Authors: José F Siqueira; Henrique S Antunes; Isabela N Rôças; Caio T C C Rachid; Flávio R F Alves Journal: PLoS One Date: 2016-09-30 Impact factor: 3.240