BACKGROUND: Acute gastroenteritis norovirus (NoV) in a country of continental dimensions like Brazil has resulted in under-reporting of the number of outbreaks, as well as the genotypes associated. OBJECTIVES: To demonstrate the role of NoV in outbreaks occurring in the State of Rio Grande do Sul, Southern Brazil, we determined its prevalence, as well as the genotypes associated, and evaluated clinical and epidemiological aspects. STUDY DESIGN: NoV investigation was carried out in rotavirus group A negative stool samples from 2265 patients from 741 outbreaks that occurred in the State of Rio Grande do Sul, Brazil, during a period of eight years (2004-2011). NoV detection and nucleotide sequencing for genotype characterization was carried by using sets of primers targeting a conservative Rd-Rp polymerase genome region and the viral capsid gene, respectively. RESULTS: NoVs were detected in 817 stool samples (36.1%) and associated with 327 outbreaks (44.1%). NoV GII.2, GII.3, GII.4, GII.6, GII.12, GII.13, GII.14, GII.15, GII.17, GII.21; and GI.1 and GI.3 were characterized. GII.4 was the most frequently detected (72.3%), with five variants identified (Asia_2003, Hunter_2004, Yerseke_2006a, Den_Haag_2006b, New Orleans_2009). This study describes the first detection of GI.1 and GII.13 and GII.15 in Brazil and demonstrates NoV winter-spring seasonality in this region of the country. CONCLUSIONS: NoVs were responsible for almost 50% of outbreaks, with about 70% of them resulting from genotype GII.4 and its variants. The seasonality observed could help health authorities to establish a system of active surveillance in order to reduce NoV impact especially in congregate settings.
BACKGROUND: Acute gastroenteritis norovirus (NoV) in a country of continental dimensions like Brazil has resulted in under-reporting of the number of outbreaks, as well as the genotypes associated. OBJECTIVES: To demonstrate the role of NoV in outbreaks occurring in the State of Rio Grande do Sul, Southern Brazil, we determined its prevalence, as well as the genotypes associated, and evaluated clinical and epidemiological aspects. STUDY DESIGN: NoV investigation was carried out in rotavirus group A negative stool samples from 2265 patients from 741 outbreaks that occurred in the State of Rio Grande do Sul, Brazil, during a period of eight years (2004-2011). NoV detection and nucleotide sequencing for genotype characterization was carried by using sets of primers targeting a conservative Rd-Rp polymerase genome region and the viral capsid gene, respectively. RESULTS: NoVs were detected in 817 stool samples (36.1%) and associated with 327 outbreaks (44.1%). NoV GII.2, GII.3, GII.4, GII.6, GII.12, GII.13, GII.14, GII.15, GII.17, GII.21; and GI.1 and GI.3 were characterized. GII.4 was the most frequently detected (72.3%), with five variants identified (Asia_2003, Hunter_2004, Yerseke_2006a, Den_Haag_2006b, New Orleans_2009). This study describes the first detection of GI.1 and GII.13 and GII.15 in Brazil and demonstrates NoV winter-spring seasonality in this region of the country. CONCLUSIONS: NoVs were responsible for almost 50% of outbreaks, with about 70% of them resulting from genotype GII.4 and its variants. The seasonality observed could help health authorities to establish a system of active surveillance in order to reduce NoV impact especially in congregate settings.
Authors: Julia Monassa Fioretti; Tulio Machado Fumian; Mônica Simões Rocha; Ingrid de Arruda Lucena Dos Santos; Filipe Aníbal Carvalho-Costa; Matheus Ribeiro de Assis; Janaina de Souza Rodrigues; José Paulo Gagliardi Leite; Marize Pereira Miagostovich Journal: Food Environ Virol Date: 2017-06-21 Impact factor: 2.778
Authors: M de Graaf; J van Beek; H Vennema; A T Podkolzin; J Hewitt; F Bucardo; K Templeton; J Mans; J Nordgren; G Reuter; M Lynch; L D Rasmussen; N Iritani; M C Chan; V Martella; K Ambert-Balay; J Vinjé; P A White; M P Koopmans Journal: Euro Surveill Date: 2015-07-02
Authors: Martin C W Chan; Nelson Lee; Tin-Nok Hung; Kirsty Kwok; Kelton Cheung; Edith K Y Tin; Raymond W M Lai; E Anthony S Nelson; Ting F Leung; Paul K S Chan Journal: Nat Commun Date: 2015-12-02 Impact factor: 14.919
Authors: Thais Alves da Costa Lamounier; Layssa Miranda de Oliveira; Brenda Rabello de Camargo; Kelly Barreto Rodrigues; Eliane Ferreira Noronha; Bergmann Morais Ribeiro; Tatsuya Nagata Journal: Braz J Microbiol Date: 2015-10-09 Impact factor: 2.476
Authors: Samya Thalita Picanço da Costa; Tulio Machado Fumian; Ian Carlos Gomes de Lima; Jones Anderson Monteiro Siqueira; Luciana Damascena da Silva; Juliana das Mercês Hernández; Maria Silvia Souza de Lucena; Tammy Kathlyn Amaral Reymão; Luana da Silva Soares; Joana D'Arc Pereira Mascarenhas; Yvone Benchimol Gabbay Journal: Mem Inst Oswaldo Cruz Date: 2017-06 Impact factor: 2.743
Authors: Tulio Machado Fumian; Juliana da Silva Ribeiro de Andrade; José Paulo Gagliardi Leite; Marize Pereira Miagostovich Journal: PLoS One Date: 2016-04-26 Impact factor: 3.240
Authors: Silvana Augusta Rodrigues Portes; Eduardo de Mello Volotão; Monica Simões Rocha; Maria Cristina Rebelo; Maria da Penha Trindade Pinheiro Xavier; Rosane Maria de Assis; Tatiana Lundgren Rose; Marize Pereira Miagostovich; José Paulo Gagliardi Leite; Filipe Anibal Carvalho-Costa Journal: Mem Inst Oswaldo Cruz Date: 2016-05-24 Impact factor: 2.743