Anton Kushnaryov1, Tomonoro Yamaguchi2, Kristen K Briggs3, Van W Wong3, Marsha Reuther1, Monica Neuman4, Victor Lin5, Robert L Sah3, Koichi Masuda2, Deborah Watson1. 1. Division of Otolaryngology-Head and Neck Surgery, University of California, San Diego, La Jolla, California, USA ; Head and Neck Surgery Section, VA San Diego Healthcare System, San Diego, California, USA. 2. Department of Orthopedic Surgery, University of California, San Diego, La Jolla, California, USA. 3. Department of Bioengineering, University of California, San Diego, La Jolla, California, USA. 4. Creighton University School of Medicine, Omaha, Nebraska, USA. 5. University of North Texas Health Sciences Center, Fort Worth, Texas, USA.
Abstract
OBJECTIVES: Evaluate safety of autogenous engineered septal neocartilage grafts.Compare properties of implanted grafts versus in vitro controls. STUDY DESIGN: Prospective, basic science. SETTING: Research laboratory. METHODS: Constructs were fabricated from septal cartilage and serum harvested from adult rabbits and then cultured in vitro or implanted on the nasal dorsum as autogenous grafts for 30 or 60 days. Rabbits were monitored for local and systemic complications. Histological, biochemical and biomechanical properties of implanted and in vitro constructs were evaluated and compared. RESULTS: No systemic or serious local complications were observed. After 30 and 60 days, implanted constructs contained more DNA (p<0.01) and less sGAG per DNA (p<0.05) when compared with in vitro controls. Confined compressive aggregate moduli were also higher in implanted constructs when compared with in vitro controls (p<0.05) and increased with longer in vivo incubation time (p<0.01). Implanted constructs displayed resorption rates of 20-45 percent. Calcium deposition in implanted constructs was observed using alizarin red histochemistry and microtomographic analyses. CONCLUSION: Autogenous engineered septal cartilage grafts were well tolerated. As seen in experiments with athymic mice, implanted constructs accumulated more DNA and less sGAG when compared with in vitro controls. Confined compressive aggregate moduli were also higher in implanted constructs. Implanted constructs displayed resorption rates similar to previously published studies using autogenous implants of native cartilage. The basis for observed calcification in implanted constructs and its effect on long-term graft efficacy is unknown at this time and will be a focus of future studies.
OBJECTIVES: Evaluate safety of autogenous engineered septal neocartilage grafts.Compare properties of implanted grafts versus in vitro controls. STUDY DESIGN: Prospective, basic science. SETTING: Research laboratory. METHODS: Constructs were fabricated from septal cartilage and serum harvested from adult rabbits and then cultured in vitro or implanted on the nasal dorsum as autogenous grafts for 30 or 60 days. Rabbits were monitored for local and systemic complications. Histological, biochemical and biomechanical properties of implanted and in vitro constructs were evaluated and compared. RESULTS: No systemic or serious local complications were observed. After 30 and 60 days, implanted constructs contained more DNA (p<0.01) and less sGAG per DNA (p<0.05) when compared with in vitro controls. Confined compressive aggregate moduli were also higher in implanted constructs when compared with in vitro controls (p<0.05) and increased with longer in vivo incubation time (p<0.01). Implanted constructs displayed resorption rates of 20-45 percent. Calcium deposition in implanted constructs was observed using alizarin red histochemistry and microtomographic analyses. CONCLUSION: Autogenous engineered septal cartilage grafts were well tolerated. As seen in experiments with athymic mice, implanted constructs accumulated more DNA and less sGAG when compared with in vitro controls. Confined compressive aggregate moduli were also higher in implanted constructs. Implanted constructs displayed resorption rates similar to previously published studies using autogenous implants of native cartilage. The basis for observed calcification in implanted constructs and its effect on long-term graft efficacy is unknown at this time and will be a focus of future studies.
Entities:
Keywords:
New Zealand White rabbit; animal model; autologous graft; cartilage tissue engineering; human septal cartilage
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