Literature DB >> 25220913

A method for evaluating nucleosome stability with a protein-binding fluorescent dye.

Hiroyuki Taguchi1, Naoki Horikoshi2, Yasuhiro Arimura1, Hitoshi Kurumizaka3.   

Abstract

Nucleosomes are extremely stable histone-DNA complexes that form the building blocks of chromatin, which accommodates genomic DNA within the nucleus. The dynamic properties of chromatin play essential roles in regulating genomic DNA functions, such as DNA replication, recombination, repair, and transcription. Histones are the protein components of nucleosomes, and their diverse modifications and variants increase the versatility of nucleosome structures and their dynamics in chromatin. Therefore, a technique to evaluate the physical properties of nucleosomes would facilitate functional studies of the various nucleosomes. In this report, we describe a convenient assay for evaluating the thermal stability of nucleosomes in vitro.
Copyright © 2014 Elsevier Inc. All rights reserved.

Keywords:  Chromatin; Histone octamer; Histone variant; Nucleosome; SYPRO Orange; Tetrasome

Mesh：

Substances：

Year:  2014        PMID： 25220913     DOI： 10.1016/j.ymeth.2014.08.019

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  27 in total

1.  Identification of the amino acid residues responsible for stable nucleosome formation by histone H3.Y.

Authors:  Tomoya Kujirai; Naoki Horikoshi; Yan Xie; Hiroyuki Taguchi; Hitoshi Kurumizaka
Journal:  Nucleus       Date:  2017-01-24       Impact factor: 4.197

2.  Time Resolved-Fluorescence Resonance Energy Transfer platform for quantitative nucleosome binding and footprinting.

Authors:  Nathaniel A Wesley; Aleksandra Skrajna; Holly C Simmons; Gabrielle R Budziszewski; Dalal N Azzam; Andrew P Cesmat; Robert K McGinty
Journal:  Protein Sci       Date:  2022-06       Impact factor: 6.993

3.  Oncohistone mutations enhance chromatin remodeling and alter cell fates.

Authors:  John D Bagert; Michelle M Mitchener; Agata L Patriotis; Barbara E Dul; Felix Wojcik; Benjamin A Nacev; Lijuan Feng; C David Allis; Tom W Muir
Journal:  Nat Chem Biol       Date:  2021-03-01       Impact factor: 16.174

Review 4.  Collaboration through chromatin: motors of transcription and chromatin structure.

Authors:  Nathan Gamarra; Geeta J Narlikar
Journal:  J Mol Biol       Date:  2021-02-05       Impact factor: 6.151

5.  Crystal structure and stable property of the cancer-associated heterotypic nucleosome containing CENP-A and H3.3.

Authors:  Yasuhiro Arimura; Kazuyoshi Shirayama; Naoki Horikoshi; Risa Fujita; Hiroyuki Taguchi; Wataru Kagawa; Tatsuo Fukagawa; Geneviève Almouzni; Hitoshi Kurumizaka
Journal:  Sci Rep       Date:  2014-11-19       Impact factor: 4.379

6.  Crystal structures of heterotypic nucleosomes containing histones H2A.Z and H2A.

Authors:  Naoki Horikoshi; Yasuhiro Arimura; Hiroyuki Taguchi; Hitoshi Kurumizaka
Journal:  Open Biol       Date:  2016-06       Impact factor: 6.411

7.  The CHD4-related syndrome: a comprehensive investigation of the clinical spectrum, genotype-phenotype correlations, and molecular basis.

Authors:  Paul A Wade; Katherine Lachlan; Karin Weiss; Hayley P Lazar; Alina Kurolap; Ariel F Martinez; Tamar Paperna; Lior Cohen; Marie F Smeland; Sandra Whalen; Solveig Heide; Boris Keren; Pauline Terhal; Melita Irving; Motoki Takaku; John D Roberts; Robert M Petrovich; Samantha A Schrier Vergano; Amy Kenney; Hanne Hove; Elizabeth DeChene; Shane C Quinonez; Estelle Colin; Alban Ziegler; Melissa Rumple; Mahim Jain; Danielle Monteil; Elizabeth R Roeder; Kimberly Nugent; Arie van Haeringen; Michael Gambello; Avni Santani; Līvija Medne; Bryan Krock; Cara M Skraban; Elaine H Zackai; Holly A Dubbs; Thomas Smol; Jamal Ghoumid; Michael J Parker; Michael Wright; Peter Turnpenny; Jill Clayton-Smith; Kay Metcalfe; Hitoshi Kurumizaka; Bruce D Gelb; Hagit Baris Feldman; Philippe M Campeau; Maximilian Muenke
Journal:  Genet Med       Date:  2019-08-07       Impact factor: 8.822

8.  Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis.

Authors:  Takashi Urahama; Akihito Harada; Kazumitsu Maehara; Naoki Horikoshi; Koichi Sato; Yuko Sato; Koji Shiraishi; Norihiro Sugino; Akihisa Osakabe; Hiroaki Tachiwana; Wataru Kagawa; Hiroshi Kimura; Yasuyuki Ohkawa; Hitoshi Kurumizaka
Journal:  Epigenetics Chromatin       Date:  2016-01-15       Impact factor: 4.954

9.  Influence of DNA methylation on positioning and DNA flexibility of nucleosomes with pericentric satellite DNA.

Authors:  Akihisa Osakabe; Fumiya Adachi; Yasuhiro Arimura; Kazumitsu Maehara; Yasuyuki Ohkawa; Hitoshi Kurumizaka
Journal:  Open Biol       Date:  2015-10       Impact factor: 6.411

10.  Structural and biochemical analyses of monoubiquitinated human histones H2B and H4.

Authors:  Shinichi Machida; Satoshi Sekine; Yuuki Nishiyama; Naoki Horikoshi; Hitoshi Kurumizaka
Journal:  Open Biol       Date:  2016-06       Impact factor: 6.411
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.