Linda M Parsons1, Marta Portela2, Nicola A Grzeschik2, Helena E Richardson3. 1. Cell Cycle and Development Laboratory, Research Division, Peter MacCallum Cancer Centre, 7 St. Andrew's Place, East Melbourne, Melbourne, VIC 3002, Australia; Department of Genetics, University of Melbourne, 1-100 Grattan Street, Parkville, Melbourne, VIC 3010, Australia. 2. Cell Cycle and Development Laboratory, Research Division, Peter MacCallum Cancer Centre, 7 St. Andrew's Place, East Melbourne, Melbourne, VIC 3002, Australia. 3. Cell Cycle and Development Laboratory, Research Division, Peter MacCallum Cancer Centre, 7 St. Andrew's Place, East Melbourne, Melbourne, VIC 3002, Australia; Sir Peter MacCallum Department of Oncology, Peter MacCallum Cancer Centre, 7 St. Andrew's Place, East Melbourne, Melbourne, VIC 3002, Australia; Department of Biochemistry and Molecular Biology, University of Melbourne, 1-100 Grattan Street, Parkville, Melbourne, VIC 3010, Australia; Department of Anatomy and Neuroscience, University of Melbourne, 1-100 Grattan Street, Parkville, Melbourne, VIC 3010, Australia. Electronic address: helena.richardson@petermac.org.
Abstract
BACKGROUND: The Drosophila melanogaster junctional neoplastic tumor suppressor, Lethal-2-giant larvae (Lgl), is a regulator of apicobasal cell polarity and tissue growth. We have previously shown in the developing Drosophila eye epithelium that, without affecting cell polarity, depletion of Lgl results in ectopic cell proliferation and blockage of developmental cell death due to deregulation of the Hippo signaling pathway. RESULTS: Here, we show that Notch signaling is increased in lgl-depleted eye tissue, independently of Lgl's function in apicobasal cell polarity. The upregulation of Notch signaling is ligand dependent and correlates with accumulation of cleaved Notch. Concomitant with higher cleaved Notch levels in lgl- tissue, early endosomes (Avalanche [Avl+]), recycling endosomes (Rab11+), early multivesicular bodies (Hrs+), and acidified vesicles, but not late endosomal markers (Car+ and Rab7+), accumulate. Colocalization studies revealed that Lgl associates with early to late endosomes and lysosomes. Upregulation of Notch signaling in lgl- tissue requires dynamin- and Rab5-mediated endocytosis and vesicle acidification but is independent of Hrs/Stam or Rab11 activity. Furthermore, Lgl regulates Notch signaling independently of the aPKC-Par6-Baz apical polarity complex. CONCLUSIONS: Altogether, our data show that Lgl regulates endocytosis to restrict vesicle acidification and prevent ectopic ligand-dependent Notch signaling. This Lgl function is independent of the aPKC-Par6-Baz polarity complex and uncovers a novel attenuation mechanism of ligand-activated Notch signaling during Drosophila eye development.
BACKGROUND: The Drosophila melanogaster junctional neoplastic tumor suppressor, Lethal-2-giant larvae (Lgl), is a regulator of apicobasal cell polarity and tissue growth. We have previously shown in the developing Drosophila eye epithelium that, without affecting cell polarity, depletion of Lgl results in ectopic cell proliferation and blockage of developmental cell death due to deregulation of the Hippo signaling pathway. RESULTS: Here, we show that Notch signaling is increased in lgl-depleted eye tissue, independently of Lgl's function in apicobasal cell polarity. The upregulation of Notch signaling is ligand dependent and correlates with accumulation of cleaved Notch. Concomitant with higher cleaved Notch levels in lgl- tissue, early endosomes (Avalanche [Avl+]), recycling endosomes (Rab11+), early multivesicular bodies (Hrs+), and acidified vesicles, but not late endosomal markers (Car+ and Rab7+), accumulate. Colocalization studies revealed that Lgl associates with early to late endosomes and lysosomes. Upregulation of Notch signaling in lgl- tissue requires dynamin- and Rab5-mediated endocytosis and vesicle acidification but is independent of Hrs/Stam or Rab11 activity. Furthermore, Lgl regulates Notch signaling independently of the aPKC-Par6-Baz apical polarity complex. CONCLUSIONS: Altogether, our data show that Lgl regulates endocytosis to restrict vesicle acidification and prevent ectopic ligand-dependent Notch signaling. This Lgl function is independent of the aPKC-Par6-Baz polarity complex and uncovers a novel attenuation mechanism of ligand-activated Notch signaling during Drosophila eye development.
Authors: Marta Portela; Liu Yang; Sayantanee Paul; Xia Li; Alexey Veraksa; Linda M Parsons; Helena E Richardson Journal: Sci Signal Date: 2018-06-05 Impact factor: 8.192
Authors: Sofia Caria; Charlene M Magtoto; Tinaz Samiei; Marta Portela; Krystle Y B Lim; Jing Yuan How; Bryce Z Stewart; Patrick O Humbert; Helena E Richardson; Marc Kvansakul Journal: J Biol Chem Date: 2018-01-29 Impact factor: 5.157
Authors: Michael A Flinn; Cécile Otten; Zachary J Brandt; Jonathan R Bostrom; Aria Kenarsary; Tina C Wan; John A Auchampach; Salim Abdelilah-Seyfried; Caitlin C O'Meara; Brian A Link Journal: Development Date: 2020-08-25 Impact factor: 6.868
Authors: Linda M Parsons; Nicola A Grzeschik; Kasun Amaratunga; Peter Burke; Leonie M Quinn; Helena E Richardson Journal: G3 (Bethesda) Date: 2017-08-07 Impact factor: 3.154