Carmen Pérez de Ciriza1, María Moreno1, Patricia Restituto1, Gorka Bastarrika2, Isabel Simón2, Inmaculada Colina3, Nerea Varo4. 1. Clinical Chemistry Department, Clínica Universidad de Navarra, Pamplona, Spain. 2. Department of Radiology, Clínica Universidad de Navarra, Pamplona, Spain. 3. Department of Internal Medicine, Clínica Universidad de Navarra, Pamplona, Spain. 4. Clinical Chemistry Department, Clínica Universidad de Navarra, Pamplona, Spain. Electronic address: nvaro@unav.es.
Abstract
CONTEXT: The relationship between osteoprotegerin (OPG) a glycoprotein related to bone metabolism and the metabolic syndrome (MS) has not been established. OBJECTIVE: The aim of this study is to evaluate OPG concentration in patients with MS and its association with subclinical atherosclerosis and coronary arterial calcification (CAC). MATERIALS/ METHODS: The study included 238 asymptomatic patients. MS was diagnosed according to the NCEP/ATPIII guidelines. OPG was measured by ELISA. All subjects underwent ultrasonography of the common carotid arteries to measure intima-media thickness (IMT) and evaluate the presence of atheroma plaques. In a subgroup (n=39) CAC was quantified by ECG-triggered cardiac computed tomography. Adipose tissue was excised from 25 patients and OPG expression by RT-PCR and immunohistochemistry was studied. RESULTS: Patients with the MS (n=60) had higher OPG than patients without (n=178) (p<0.05). OPG correlated with IMT (r=0.2, p=0.005) and patients with atheroma plaques had higher OPG (p=0.008) and also those with coronary artery calcification (p<0.05). OPG expression was confirmed in adipose tissue (n=12) and the expression was significantly higher in patients with MS than in those without (p=0.003). CONCLUSIONS: This study shows that OPG may potentially be a biomarker for cardiovascular risk/damage in the MS and identifies adipose tissue as a potential source of OPG.
CONTEXT: The relationship between osteoprotegerin (OPG) a glycoprotein related to bone metabolism and the metabolic syndrome (MS) has not been established. OBJECTIVE: The aim of this study is to evaluate OPG concentration in patients with MS and its association with subclinical atherosclerosis and coronary arterial calcification (CAC). MATERIALS/ METHODS: The study included 238 asymptomatic patients. MS was diagnosed according to the NCEP/ATPIII guidelines. OPG was measured by ELISA. All subjects underwent ultrasonography of the common carotid arteries to measure intima-media thickness (IMT) and evaluate the presence of atheroma plaques. In a subgroup (n=39) CAC was quantified by ECG-triggered cardiac computed tomography. Adipose tissue was excised from 25 patients and OPG expression by RT-PCR and immunohistochemistry was studied. RESULTS:Patients with the MS (n=60) had higher OPG than patients without (n=178) (p<0.05). OPG correlated with IMT (r=0.2, p=0.005) and patients with atheroma plaques had higher OPG (p=0.008) and also those with coronary artery calcification (p<0.05). OPG expression was confirmed in adipose tissue (n=12) and the expression was significantly higher in patients with MS than in those without (p=0.003). CONCLUSIONS: This study shows that OPG may potentially be a biomarker for cardiovascular risk/damage in the MS and identifies adipose tissue as a potential source of OPG.
Authors: Nienke M S Golüke; Marit A Schoffelmeer; Annemarieke De Jonghe; Mariëlle H Emmelot-Vonk; Pim A De Jong; Huiberdina L Koek Journal: Bone Rep Date: 2022-06-18
Authors: Miguel Bernardes; Tiago S Vieira; Maria João Martins; Raquel Lucas; Lúcia Costa; Jorge G Pereira; Francisco Ventura; Elisabete Martins Journal: Biomed Res Int Date: 2017-05-03 Impact factor: 3.411
Authors: Carmen G Barbu; Andreea L Arsene; Suzana Florea; Alice Albu; Anca Sirbu; Sorina Martin; Alina C Nicolae; George T A Burcea-Dragomiroiu; Daniela E Popa; Bruno S Velescu; Ion B Dumitrescu; Niculina Mitrea; Doina Draganescu; Dumitru Lupuliasa; Demetrios A Spandidos; Aristides M Tsatsakis; Cristina M Dragoi; Simona Fica Journal: Mol Med Rep Date: 2017-08-28 Impact factor: 2.952