Chi-Jr Hung1, Hsin-I Hsu2, Chi-Chang Lin3, Tsui-Hsien Huang1, Buor-Chang Wu1, Chia-Tze Kao4, Ming-You Shie5. 1. School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Stomatology, Chung Shan University Hospital, Taichung City, Taiwan. 2. Institute of Oral Science, Chung Shan Medical University, Taichung City, Taiwan. 3. Department of Chemical and Materials Engineering, Tunghai University, Taichung City, Taiwan. 4. School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan; Department of Stomatology, Chung Shan University Hospital, Taichung City, Taiwan. Electronic address: ctk@csmu.edu.tw. 5. School of Dentistry, Chung Shan Medical University, Taichung City, Taiwan. Electronic address: eviltacasi@gmail.com.
Abstract
INTRODUCTION: It has been proved that integrin αv activity is related to cell proliferation, differentiation, migration, and organ development. However, the biological functions of integrin αv in human dental pulp cells (hDPCs) cultured on silicate-based materials have not been explored. The aim of this study was to investigate the role of integrin αv in the proliferation and odontogenic differentiation of hDPCs cultured with the effect of calcium silicate (CS) cement and β-tricalcium phosphate (TCP) cement. METHODS: In this study, hDPCs were cultured on CS and TCP materials, and we evaluated fibronectin (FN) secretion and integrin αv expression during the cell attachment stage. After small interfering RNA transfection targeting integrin αv, the proliferation and odontogenesis differentiation behavior of hDPCs were analyzed. RESULTS: The results indicate that CS releases Si ion-increased FN secretion and adsorption, which promote cell attachment more effectively than TCP. The CS cement facilitates FN and αv subintegrin expression. However, the FN adsorption and integrin expression of TCP are similar to that observed in the control dish. Integrin αv small interfering RNA inhibited odontogenic differentiation of hDPCs with the decreased formation of mineralized nodules on CS. It also down-regulated the protein expression of multiple markers of odontogenesis and the expression of dentin sialophosphoprotein protein. CONCLUSIONS: These results establish composition-dependent differences in integrin binding and its effectiveness as a mechanism regulating cellular responses to biomaterial surface.
INTRODUCTION: It has been proved that integrin αv activity is related to cell proliferation, differentiation, migration, and organ development. However, the biological functions of integrin αv in human dental pulp cells (hDPCs) cultured on silicate-based materials have not been explored. The aim of this study was to investigate the role of integrin αv in the proliferation and odontogenic differentiation of hDPCs cultured with the effect of calcium silicate (CS) cement and β-tricalcium phosphate (TCP) cement. METHODS: In this study, hDPCs were cultured on CS and TCP materials, and we evaluated fibronectin (FN) secretion and integrin αv expression during the cell attachment stage. After small interfering RNA transfection targeting integrin αv, the proliferation and odontogenesis differentiation behavior of hDPCs were analyzed. RESULTS: The results indicate that CS releases Si ion-increased FN secretion and adsorption, which promote cell attachment more effectively than TCP. The CS cement facilitates FN and αv subintegrin expression. However, the FN adsorption and integrin expression of TCP are similar to that observed in the control dish. Integrin αv small interfering RNA inhibited odontogenic differentiation of hDPCs with the decreased formation of mineralized nodules on CS. It also down-regulated the protein expression of multiple markers of odontogenesis and the expression of dentin sialophosphoprotein protein. CONCLUSIONS: These results establish composition-dependent differences in integrin binding and its effectiveness as a mechanism regulating cellular responses to biomaterial surface.
Authors: Ivan López-González; Camilo Zamora-Ledezma; María Isabel Sanchez-Lorencio; Elena Tristante Barrenechea; José Antonio Gabaldón-Hernández; Luis Meseguer-Olmo Journal: Int J Mol Sci Date: 2021-10-18 Impact factor: 5.923