Literature DB >> 25218385

Multiplexed miRNA fluorescence in situ hybridization for formalin-fixed paraffin-embedded tissues.

Neil Renwick1, Pavol Cekan, Claudia Bognanni, Thomas Tuschl.   

Abstract

Multiplexed miRNA fluorescence in situ hybridization (miRNA FISH) is an advanced method for visualizing differentially expressed miRNAs, together with other reference RNAs, in archival tissues. Some miRNAs are excellent disease biomarkers due to their abundance and cell-type specificity. However, these short RNA molecules are difficult to visualize due to loss by diffusion, probe mishybridization, and signal detection and signal amplification issues. Here, we describe a reliable and adjustable method for visualizing and normalizing miRNA signals in formalin-fixed paraffin-embedded (FFPE) tissue sections.

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Year:  2014        PMID： 25218385      PMCID： PMC4309551          DOI： 10.1007/978-1-4939-1459-3_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification.

Authors:  A H Hopman; F C Ramaekers; E J Speel
Journal:  J Histochem Cytochem       Date:  1998-06       Impact factor: 2.479

2.  Multicolor microRNA FISH effectively differentiates tumor types.

Authors:  Neil Renwick; Pavol Cekan; Paul A Masry; Sean E McGeary; Jason B Miller; Markus Hafner; Zhen Li; Aleksandra Mihailovic; Pavel Morozov; Miguel Brown; Tasos Gogakos; Mehrpouya B Mobin; Einar L Snorrason; Harriet E Feilotter; Xiao Zhang; Clifford S Perlis; Hong Wu; Mayte Suárez-Fariñas; Huichen Feng; Masahiro Shuda; Patrick S Moore; Victor A Tron; Yuan Chang; Thomas Tuschl
Journal:  J Clin Invest       Date:  2013-06       Impact factor: 14.808

3.  miRNA in situ hybridization in formaldehyde and EDC-fixed tissues.

Authors:  John T G Pena; Cherin Sohn-Lee; Sara H Rouhanifard; Janos Ludwig; Markus Hafner; Aleksandra Mihailovic; Cindy Lim; Daniel Holoch; Philipp Berninger; Mihaela Zavolan; Thomas Tuschl
Journal:  Nat Methods       Date:  2009-01-11       Impact factor: 28.547

4.  Barcoded cDNA library preparation for small RNA profiling by next-generation sequencing.

Authors:  Markus Hafner; Neil Renwick; Thalia A Farazi; Aleksandra Mihailović; John T G Pena; Thomas Tuschl
Journal:  Methods       Date:  2012-08-07       Impact factor: 3.608
  4 in total
  6 in total

1.  A non-radioactive, improved PAR-CLIP and small RNA cDNA library preparation protocol.

Authors:  Dimitrios G Anastasakis; Alexis Jacob; Parthena Konstantinidou; Kazuyuki Meguro; Duncan Claypool; Pavol Cekan; Astrid D Haase; Markus Hafner
Journal:  Nucleic Acids Res       Date:  2021-05-07       Impact factor: 16.971

Review 2.  Small RNA Detection by in Situ Hybridization Methods.

Authors:  Martyna O Urbanek; Anna U Nawrocka; Wlodzimierz J Krzyzosiak
Journal:  Int J Mol Sci       Date:  2015-06-10       Impact factor: 5.923

3.  Upregulation of microRNA-106b is associated with poor prognosis in hepatocellular carcinoma.

Authors:  Bin-Kui Li; Pin-Zhu Huang; Ji-Liang Qiu; Ya-Di Liao; Jian Hong; Yun-Fei Yuan
Journal:  Diagn Pathol       Date:  2014-12-03       Impact factor: 2.644

4.  Head and neck squamous cancer progression is marked by CLIC4 attenuation in tumor epithelium and reciprocal stromal upregulation of miR-142-3p, a novel post-transcriptional regulator of CLIC4.

Authors:  Brandi L Carofino; Kayla M Dinshaw; Pui Yan Ho; Christophe Cataisson; Aleksandra M Michalowski; Andrew Ryscavage; Addie Alkhas; Nathan W Wong; Vishal Koparde; Stuart H Yuspa
Journal:  Oncotarget       Date:  2019-12-31

5.  A novel approach for microRNA in situ hybridization using locked nucleic acid probes.

Authors:  Isabella W Paulsen; Michael Bzorek; Jesper Olsen; Birgitte Grum-Schwensen; Jesper T Troelsen; Ole B Pedersen
Journal:  Sci Rep       Date:  2021-02-24       Impact factor: 4.379

Review 6.  Simultaneous detection of the subcellular localization of RNAs and proteins in cultured cells by combined multicolor RNA-FISH and IF.

Authors:  Cindy Meyer; Aitor Garzia; Thomas Tuschl
Journal:  Methods       Date:  2016-09-21       Impact factor: 3.608
  6 in total

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