Literature DB >> 25218111

Evaluation of Borrelia real time PCR DNA targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR.

Bertie H C G M de Leeuw1, Boulos Maraha2, Leonie Hollemans2, Hein Sprong3, Afke H Brandenburg4, Pieter J Westenend5, Johannes G Kusters6.   

Abstract

Borrelia burgdorferi non-sensu lato (s.l.) strains occurred in the Netherlands. A multiplex OspA, FlaB, IGS real time PCR was compared to 16S rRNA/rDNA RT-qPCR with lower average Cycle threshold (Ct) and LOD on strain dilutions. Multiplexing increased sensitivity on CSF samples (n=74), distinguishing B. burgdorferi s.l. from non-s.l. strains.
Copyright © 2014 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Borrelia; Lyme; Real time PCR

Mesh：

Substances：

Year:  2014        PMID： 25218111     DOI： 10.1016/j.mimet.2014.09.001

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  4 in total

1.  Direct Molecular Detection and Genotyping of Borrelia burgdorferi Sensu Lato in Cerebrospinal Fluid of Children with Lyme Neuroborreliosis.

Authors:  Bjørn Barstad; Hanne Quarsten; Dag Tveitnes; Sølvi Noraas; Ingvild S Ask; Maryam Saeed; Franziskus Bosse; Grete Vigemyr; Ilka Huber; Knut Øymar
Journal:  J Clin Microbiol       Date:  2018-04-25       Impact factor: 5.948

2.  Co-infections with multiple pathogens in natural populations of Ixodes persulcatus ticks in Mongolia.

Authors:  Ekaterina K Lagunova; Natalia A Liapunova; Davaakhu Tuul; Gerechuluun Otgonsuren; Davaadorj Nomin; Nyamdorj Erdenebat; Davaajav Abmed; Galina A Danchinova; Kozue Sato; Hiroki Kawabata; Maxim A Khasnatinov
Journal:  Parasit Vectors       Date:  2022-06-28       Impact factor: 4.047

Review 3.  Quantitative multiplexed strategies for human Lyme disease serological testing.

Authors:  Eunice Chou; Armond Minor; Nathaniel C Cady
Journal:  Exp Biol Med (Maywood)       Date:  2021-04-01

4.  Molecular detection of Borrelia burgdorferi sensu lato - An analytical comparison of real-time PCR protocols from five different Scandinavian laboratories.

Authors:  Malin Lager; Maximilian Faller; Peter Wilhelmsson; Vivian Kjelland; Åshild Andreassen; Rimtas Dargis; Hanne Quarsten; Ram Dessau; Volker Fingerle; Gabriele Margos; Sølvi Noraas; Katharina Ornstein; Ann-Cathrine Petersson; Andreas Matussek; Per-Eric Lindgren; Anna J Henningsson
Journal:  PLoS One       Date:  2017-09-22       Impact factor: 3.240
  4 in total

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