Yazhen Yuan1, Zhuan Wen1, Yongqing Guan2, Yanhua Sun3, Jie Yang4, Xiaohui Fan5, Xinzhi Yang1, Rui Liu1. 1. Department of Ophthalmology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China 050011. 2. Department of Ophthalmology, the Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China 050011. Electronic address: Yongqingguan@hotmail.com. 3. Shijiazhuang Aier Hospital Department of Ophthalmology, Hebei Province, China 050000. 4. Department of Ophthalmology, the People's Hospital of Binzhou, Shandong Province, China 256600. 5. Department of Ophthalmology, Affiliated Hospital of Chengde Medical College, Hebei Province, China 067000.
Abstract
OBJECTIVE: To investigate how VEGF-634G/C and VEGF-460C/T SNPs are related to diabetic retinopathy (DR) in Han Chinese subjects from the Shijiazhuang region of China. METHODS: Totally 376 DM cases were divided into non-proliferative diabetic retinopathy (NPDR) group (n=124), proliferative diabetic retinopathy (PDR) group (n=108), and diabetes without retinopathy (DWR) group (n=144). PCR/LDRwas utilised to detect and assess the genotypes and allele distribution frequencies at the VEGF-634G/C and VEGF-460C/T loci in each group. RESULTS: The differences between NPDR, PDR and DWR groups were not significant in genotypes and allele distribution frequencies at VEGF-634G/C locus (P>0.05). But there were significant differences between NPDR and DWR groups in genotypes (P=0.013) and allele distribution frequencies (P=0.002) at VEGF-460C/T locus, at which CT+CC genotypes were associated with a reduced risk of developing NPDR. There were no significant differences in genotypes (P=0.759) or allele distribution frequencies (P=0.433) at VEGF-460C/T locus between PDR and DWR groups. CONCLUSIONS: Among Chinese Han individuals with type-2 DM, polymorphism -634G/C of the VEGF gene was not correlated with NPDR or PDR; however, polymorphism-460C/T of the VEGF gene was correlated with NPDR, and C allele was associated with lower NPDR risk than T allele.
OBJECTIVE: To investigate how VEGF-634G/C and VEGF-460C/T SNPs are related to diabetic retinopathy (DR) in Han Chinese subjects from the Shijiazhuang region of China. METHODS: Totally 376 DM cases were divided into non-proliferative diabetic retinopathy (NPDR) group (n=124), proliferative diabetic retinopathy (PDR) group (n=108), and diabetes without retinopathy (DWR) group (n=144). PCR/LDRwas utilised to detect and assess the genotypes and allele distribution frequencies at the VEGF-634G/C and VEGF-460C/T loci in each group. RESULTS: The differences between NPDR, PDR and DWR groups were not significant in genotypes and allele distribution frequencies at VEGF-634G/C locus (P>0.05). But there were significant differences between NPDR and DWR groups in genotypes (P=0.013) and allele distribution frequencies (P=0.002) at VEGF-460C/T locus, at which CT+CC genotypes were associated with a reduced risk of developing NPDR. There were no significant differences in genotypes (P=0.759) or allele distribution frequencies (P=0.433) at VEGF-460C/T locus between PDR and DWR groups. CONCLUSIONS: Among Chinese Han individuals with type-2 DM, polymorphism -634G/C of the VEGF gene was not correlated with NPDR or PDR; however, polymorphism-460C/T of the VEGF gene was correlated with NPDR, and C allele was associated with lower NPDR risk than T allele.