PURPOSE: To facilitate epiretinal or inner limiting membrane peeling, dyes like Indocyanine Green (ICG) as well as Trypan Blue (TB) were used so far. However, toxic effects on the retina were described for both dyes. The aim of our study was to investigate the effects of a novel vital dye Acid violet-17 (AV-17) on retinal histology and function to assess a possible application in vitreo-retinal surgery. METHODS: AV-17 was dissolved in a solvent with heavy water. An electroretinogram was recorded on perfused bovine retina. After reaching stable b-wave amplitudes, AV-17 (0.125-0.5 mg/ml) or the solvent was applied epiretinally for 30-300 seconds. The b-wave amplitudes were recorded before, during, and after treatment. Cultures of bovine retina were incubated for 30 or 300 seconds with the dye or solvent and processed for live/dead staining, immunohistochemistry, and immunoblotting. RESULTS: Reductions of the b-wave amplitudes were observed directly after the exposure to AV-17, which were rapidly and completely reversible within the recovery period for all exposure times at the concentrations of 0.125 and 0.25 mg/ml as opposed to the partial recovery after exposure to 0.5 mg/ml. A high degree of damage in the ganglion cell layer (GCL) and glial reactivity were detected at the concentrations of 0.25 and 0.5 mg/ml but not after exposure to lower concentrations or the solvent. CONCLUSION: Application of AV-17 at a concentration of up to 0.125 mg/ml was well tolerated in terms of retinal function, survival in the GCL, and glial reactivity whereas higher concentrations are not recommended.
PURPOSE: To facilitate epiretinal or inner limiting membrane peeling, dyes like Indocyanine Green (ICG) as well as Trypan Blue (TB) were used so far. However, toxic effects on the retina were described for both dyes. The aim of our study was to investigate the effects of a novel vital dye Acid violet-17 (AV-17) on retinal histology and function to assess a possible application in vitreo-retinal surgery. METHODS:AV-17 was dissolved in a solvent with heavy water. An electroretinogram was recorded on perfused bovine retina. After reaching stable b-wave amplitudes, AV-17 (0.125-0.5 mg/ml) or the solvent was applied epiretinally for 30-300 seconds. The b-wave amplitudes were recorded before, during, and after treatment. Cultures of bovine retina were incubated for 30 or 300 seconds with the dye or solvent and processed for live/dead staining, immunohistochemistry, and immunoblotting. RESULTS: Reductions of the b-wave amplitudes were observed directly after the exposure to AV-17, which were rapidly and completely reversible within the recovery period for all exposure times at the concentrations of 0.125 and 0.25 mg/ml as opposed to the partial recovery after exposure to 0.5 mg/ml. A high degree of damage in the ganglion cell layer (GCL) and glial reactivity were detected at the concentrations of 0.25 and 0.5 mg/ml but not after exposure to lower concentrations or the solvent. CONCLUSION: Application of AV-17 at a concentration of up to 0.125 mg/ml was well tolerated in terms of retinal function, survival in the GCL, and glial reactivity whereas higher concentrations are not recommended.
Authors: Peter Szurman; Radoslaw Kaczmarek; Martin S Spitzer; Gesine B Jaissle; Patrice Decker; Salvatore Grisanti; Sigrid Henke-Fahle; Sabine Aisenbrey; Karl U Bartz-Schmidt Journal: Exp Eye Res Date: 2006-05-08 Impact factor: 3.467
Authors: Andreas Bringmann; Thomas Pannicke; Jens Grosche; Mike Francke; Peter Wiedemann; Serguei N Skatchkov; Neville N Osborne; Andreas Reichenbach Journal: Prog Retin Eye Res Date: 2006-07-12 Impact factor: 21.198
Authors: Kai Januschowski; Sebastian Mueller; Martin S Spitzer; Charlotte Schramm; Deshka Doycheva; Karl-Ulrich Bartz-Schmidt; Peter Szurman Journal: Graefes Arch Clin Exp Ophthalmol Date: 2012-03-28 Impact factor: 3.117
Authors: Matthias Lüke; Kai Januschowski; Julia Beutel; Max Warga; Salvatore Grisanti; Swaantje Peters; Toni Schneider; Christoph Lüke; Karl Ulrich Bartz-Schmidt; Peter Szurman Journal: Exp Eye Res Date: 2008-04-23 Impact factor: 3.467
Authors: Fernando M Penha; Marianne Pons; Elaine Fiod Costa; Nilana Meza Tenório Barros; Eduardo B Rodrigues; Emmerson Badaró Cardoso; Eduardo Dib; Mauricio Maia; Maria E Marin-Castaño; Michel Eid Farah Journal: PLoS One Date: 2013-05-10 Impact factor: 3.240
Authors: Sebastian Mueller; Carlo Krupp; Sven Schnichels; Johanna Hofmann; Martin Spitzer; Karl Ulrich Bartz-Schmidt; Peter Szurman; Kai Januschowski Journal: Graefes Arch Clin Exp Ophthalmol Date: 2019-02-20 Impact factor: 3.117