| Literature DB >> 25214112 |
Lee Suan Chua1, Norliza Abdul Latiff2, Sze Yean Lee2, Chew Tin Lee3, Mohamad Roji Sarmidi2, Ramlan Abdul Aziz2.
Abstract
Both total phenolic content (TPC) and total flavonoid content (TFC) of Labisia pumila extracts were determined spectrophotometrically. L. pumila leaves extracted in 60% methanol (MeOH) were fractionated on C18 cartridge and the antioxidant property of each fraction was determined by measuring free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity. The 40% MeOH fraction exhibited the highest scavenging activity. Nine flavonols (quercetin, myricetin and kaempferol), two flavanols (catechin and epigallocatechin) and nine phenolic acids were identified from this active fraction by UPLC-ESI-MS/MS, and confirmed by comparison with the mass spectra of standard aglycones, theoretical fragments generated from MS Fragmenter software, and literature values.Entities:
Keywords: Flavanols; Flavonols; Labisia pumila; Phenolic acids; UPLC–ESI-MS/MS
Year: 2011 PMID: 25214112 DOI: 10.1016/j.foodchem.2011.01.122
Source DB: PubMed Journal: Food Chem ISSN: 0308-8146 Impact factor: 7.514