Spectrofluorimetric determination of buparvaquone in biological fluids, food samples and a pharmaceutical formulation by using terbium-deferasirox probe.

A simple spectrofluorimetric method is described for the determination of buparvaquone (BPQ), based on its quenching effect on the fluorescence intensity of Tb(3+)-deferasirox (DFX) complex as a fluorescent probe. The excitation and emission wavelengths were 328 and 545nm, respectively. The optimum conditions for determination of BPQ were investigated considering the effects of various affecting parameters. The variations in fluorescence intensity of the system showed a good linear relationship with the concentration of BPQ in the range of 10-1500μgL(-1), its correlation coefficient was 0.999 with the detection and quantification limits of 1.1 and 3.4μgL(-1), respectively. Linearity, reproducibility, recovery, limits of detection and quantification made the method suitable for BPQ assay in biological fluids, meat, dairy products and BPQ parenteral solutions (vials). The method was applied to real samples of serum and milk of three cows receiving BPQ.