Thomas M Benkoe1, Thomas P Mechtler2, Mario Pones3, Andrea-Romana Prusa4, Katrin Klebermass-Schrehof4, Winfried Rebhandl3, David C Kasper2. 1. Department of Pediatric Surgery, Medical University of Vienna, Waehringerguertel 18-20, 1090 Vienna, Austria. Electronic address: thomas.benkoe@meduniwien.ac.at. 2. Department of Pediatrics and Adolescent Medicine, Research Core Unit for Pediatric Biochemistry and Analytics, Medical University of Vienna, Waehringerguertel 18-20, 1090 Vienna, Austria. 3. Department of Pediatric Surgery, Medical University of Vienna, Waehringerguertel 18-20, 1090 Vienna, Austria. 4. Department of Pediatrics and Adolescent Medicine, Division of Neonatology, Pediatric Intensive Care and Neuropediatrics, Medical University of Vienna, Waehringerguertel 18-20, 1090 Vienna, Austria.
Abstract
BACKGROUND: Intestinal ischemia plays a major role in the pathogenesis of necrotizing enterocolitis (NEC). The diagnosis of intestinal ischemia would be highly desirable, as it is impossible to achieve with the current diagnostic regimes. Preliminary data from an animal NEC model indicate a possible correlation between the plasma activity of the lysosomal enzyme beta-glucosidase and intestinal ischemia. METHODS: In this case-control study the plasma activities of six different lysosomal enzymes were detected by high-performance liquid-chromatography tandem mass-spectrometry in 15 infants with NEC and compared to 18 controls. RESULTS: The plasma activities of β-glucosidase (ABG), α-glucosidase (GAA), and galactocerebrosidase (GALC) were significantly higher in the NEC group compared with controls (ABG, p=0.009; GAA, p<0.001; GALC, p<0.001). GAA and GALC showed the highest diagnostic value with areas under the curve of 0.91 and 0.87. CONCLUSIONS: We identified GAA and GALC as new promising biomarkers for gut wall integrity in infants with NEC, and report first results on the plasma activity of ABG. The present study supports the hypothesis that the plasma activity of ABG might serve as a marker of intestinal ischemia in NEC. The identification of intestinal ischemia could facilitate early discrimination of infants at risk for NEC from infants with benign gastrointestinal disorders.
BACKGROUND:Intestinal ischemia plays a major role in the pathogenesis of necrotizing enterocolitis (NEC). The diagnosis of intestinal ischemia would be highly desirable, as it is impossible to achieve with the current diagnostic regimes. Preliminary data from an animal NEC model indicate a possible correlation between the plasma activity of the lysosomal enzyme beta-glucosidase and intestinal ischemia. METHODS: In this case-control study the plasma activities of six different lysosomal enzymes were detected by high-performance liquid-chromatography tandem mass-spectrometry in 15 infants with NEC and compared to 18 controls. RESULTS: The plasma activities of β-glucosidase (ABG), α-glucosidase (GAA), and galactocerebrosidase (GALC) were significantly higher in the NEC group compared with controls (ABG, p=0.009; GAA, p<0.001; GALC, p<0.001). GAA and GALC showed the highest diagnostic value with areas under the curve of 0.91 and 0.87. CONCLUSIONS: We identified GAA and GALC as new promising biomarkers for gut wall integrity in infants with NEC, and report first results on the plasma activity of ABG. The present study supports the hypothesis that the plasma activity of ABG might serve as a marker of intestinal ischemia in NEC. The identification of intestinal ischemia could facilitate early discrimination of infants at risk for NEC from infants with benign gastrointestinal disorders.
Authors: José Luis Gómez-Chaparro Moreno; Alejandro Rodríguez Torronteras; María Dolores Ruiz González; Lucía Izquierdo Palomares; Daniel Bonilla Valverde; Julia Ruiz Laguna; Alfonso Delgado Rubio; Juan López-Barea Journal: Eur J Pediatr Date: 2016-04-27 Impact factor: 3.183
Authors: Chris H Hill; Agnete H Viuff; Samantha J Spratley; Stéphane Salamone; Stig H Christensen; Randy J Read; Nigel W Moriarty; Henrik H Jensen; Janet E Deane Journal: Chem Sci Date: 2015-03-30 Impact factor: 9.825