| Literature DB >> 25204266 |
Linda Ahonen1, Florian B R Maire2, Mari Savolainen3, Jaakko Kopra4, Rob J Vreeken5, Thomas Hankemeier6, Timo Myöhänen7, Petri Kylli8, Risto Kostiainen9.
Abstract
We have developed an ultra-high-performance liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometric (UHPLC-APPI-MS/MS) method for the simultaneous quantitative analyses of several oxysterols and vitamin D metabolites in mouse brain and cell line samples. An UHPLC-APPI-high resolution mass spectrometric (UHPLC-APPI-HRMS) method that uses a quadrupole-time of flight mass spectrometer was also developed for confirmatory analysis and for the identification of non-targeted oxysterols. Both methods showed good quantitative performance. Furthermore, APPI provides high ionization efficiency for determining oxysterols and vitamin D related compounds without the time consuming derivatization step needed in the conventionally used electrospray ionization method to achieve acceptable sensitivity. Several oxysterols were quantified in mouse brain and cell line samples. Additionally, 25-hydroxyvitamin D3 was detected in mouse brain samples for the first time.Entities:
Keywords: Atmospheric pressure photoionization; Cell line samples; Liquid chromatography–mass spectrometry; Mouse brain samples; Oxysterols; Vitamin D
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Year: 2014 PMID： 25204266 DOI： 10.1016/j.chroma.2014.08.088
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759