Sebastiano Cimino1, Vincenzo Favilla1, Giorgio Ivan Russo2, Fabio Galvano3, Giovanni Li Volti4, Ignazio Barbagallo3, Salvatore Vincenzo Giofrè5, Nicolantonio D'Orazio6, Alessandro DI Rosa1, Massimo Madonia7, Giuseppe Morgia1. 1. Department of Urology, University of Catania, Catania, Italy. 2. Department of Urology, University of Catania, Catania, Italy giorgioivan@virgilio.it. 3. Department of Biological Chemistry, Medical Chemistry and Molecular Biology, University of Catania, Catania, Italy. 4. Department of Biological Chemistry, Medical Chemistry and Molecular Biology, University of Catania, Catania, Italy Department of Stress Biology, Epigenetic and Biomarkers, Euro Mediterranean Institute of Science and Technology, Palermo, Italy. 5. Department of Pharmaceutical Sciences and Health Products, University of Messina, Messina, Italy. 6. Human and Clinical Nutrition Unit, Department of Biomedical Science, University of Chieti, Italy. 7. Department of Urology, University of Sassari, Sassari, Italy.
Abstract
OBJECTIVE: To investigate the role of body composition and oxidative stress measured by total thiol groups (TTG) levels in prostate specimens of patients affected by benign prostatic hyperplasia (BPH) or prostate cancer (PCa). PATIENTS AND METHODS: From January 2011 to January 2013, a cohort of 150 consecutive male patients who underwent first prostate biopsy were enrolled. Twelve-core needle biopsy was performed as standard procedure, while twelve more needle tissue cores matched with the previous group were also collected for glutathione determination. After definitive diagnosis, measurement of glutathione was performed in the correspondent one matched prostatic sample where PCa or BPH were identified. A day after the prostatic biopsy, body composition was estimated by air plethysmography (BOD POD®). RESULTS: A significant difference of TTG was observed in BPH and PCa patients; 34 nanomole (nmol) reagent sulfihydrylc (RSH)/ mg protein vs. 1.1 nmol RSH/ mg protein respectively (p<0.05). In BPH patients, a negative correlation was found between TTG and age (r=-0.46; p<0.05), while, in PCa patients, a positive correlation was observed between TTG and fat mass (FM) (r=0.76; p<0.01) and waist circumference (WC) (r=0.49; p<0.05). Multivariate linear regression analysis showed TTG to be negatively associated with age (β-coefficient=-0.4; p<0.05) in BPH patients and positively with FM (β-coefficient=3.4; p<0.01) and WC (β-coefficient=2.7; p<0.05) in PCa patients. CONCLUSION: Aging determines a progressive reduction of TTG in BPH patients, while in PCa subjects glutathione concentrations are significantly lower and FM and WC are associated with an unbalance of its levels. Copyright
OBJECTIVE: To investigate the role of body composition and oxidative stress measured by total thiol groups (TTG) levels in prostate specimens of patients affected by benign prostatic hyperplasia (BPH) or prostate cancer (PCa). PATIENTS AND METHODS: From January 2011 to January 2013, a cohort of 150 consecutive male patients who underwent first prostate biopsy were enrolled. Twelve-core needle biopsy was performed as standard procedure, while twelve more needle tissue cores matched with the previous group were also collected for glutathione determination. After definitive diagnosis, measurement of glutathione was performed in the correspondent one matched prostatic sample where PCa or BPH were identified. A day after the prostatic biopsy, body composition was estimated by air plethysmography (BOD POD®). RESULTS: A significant difference of TTG was observed in BPH and PCa patients; 34 nanomole (nmol) reagent sulfihydrylc (RSH)/ mg protein vs. 1.1 nmol RSH/ mg protein respectively (p<0.05). In BPH patients, a negative correlation was found between TTG and age (r=-0.46; p<0.05), while, in PCa patients, a positive correlation was observed between TTG and fat mass (FM) (r=0.76; p<0.01) and waist circumference (WC) (r=0.49; p<0.05). Multivariate linear regression analysis showed TTG to be negatively associated with age (β-coefficient=-0.4; p<0.05) in BPH patients and positively with FM (β-coefficient=3.4; p<0.01) and WC (β-coefficient=2.7; p<0.05) in PCa patients. CONCLUSION: Aging determines a progressive reduction of TTG in BPH patients, while in PCa subjects glutathione concentrations are significantly lower and FM and WC are associated with an unbalance of its levels. Copyright
Authors: Sarah A Purcell; Camila L P Oliveira; Michelle Mackenzie; Paula Robson; John D Lewis; Carla M Prado Journal: Adv Nutr Date: 2022-08-01 Impact factor: 11.567
Authors: ElShaddai Z White; Nakea M Pennant; Jada R Carter; Ohuod Hawsawi; Valerie Odero-Marah; Cimona V Hinton Journal: Sci Rep Date: 2020-07-27 Impact factor: 4.379