Sung-Ja Ahn1, Chan Choi2, Yoo-Duk Choi3, Young-Chul Kim4, Kyu-Sik Kim4, In-Jae Oh4, Hee-Jung Ban4, Mee-Sun Yoon1, Taek-Keun Nam1, Jae-Uk Jeong1, Joo-Young Song1, Woong-Ki Chung1. 1. Department of Radiation Oncology, Chonnam National University Medical School, Gwangju, Republic of Korea. 2. Department of Pathology, Chonnam National University Medical School, Gwangju, Republic of Korea cchoi@chonnam.ac.kr. 3. Department of Pathology, Chonnam National University Medical School, Gwangju, Republic of Korea. 4. Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Republic of Korea.
Abstract
BACKGROUND: To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation. MATERIALS AND METHODS: Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray. RESULTS: Four established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines. CONCLUSION: The major gene expression changes during irradiation are related to WNT signaling pathway. Copyright
BACKGROUND: To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation. MATERIALS AND METHODS:Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray. RESULTS: Four established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines. CONCLUSION: The major gene expression changes during irradiation are related to WNT signaling pathway. Copyright
Authors: Georg Emons; Melanie Spitzner; Sebastian Reineke; Janneke Möller; Noam Auslander; Frank Kramer; Yue Hu; Tim Beissbarth; Hendrik A Wolff; Margret Rave-Fränk; Elisabeth Heßmann; Jochen Gaedcke; B Michael Ghadimi; Steven A Johnsen; Thomas Ried; Marian Grade Journal: Mol Cancer Res Date: 2017-08-15 Impact factor: 5.852
Authors: Ruben S A Goedegebuure; Esther A Kleibeuker; Francesca M Buffa; Kitty C M Castricum; Syed Haider; Iris A Schulkens; Luuk Ten Kroode; Jaap van den Berg; Maarten A J M Jacobs; Anne-Marie van Berkel; Nicole C T van Grieken; Sarah Derks; Ben J Slotman; Henk M W Verheul; Adrian L Harris; Victor L Thijssen Journal: J Exp Clin Cancer Res Date: 2021-05-08