BACKGROUND: Branched chain amino acid (BCAA) dietary supplementation inhibits activation of the insulin-like growth factor (IGF)/IGF-I receptor (IGF-IR) axis in diabetic animal models. However, the in vitro effect of BCAA on human cancer cell lines under hyper-insulinemic conditions remains unclear. MATERIALS AND METHODS: Colon (HCT-116) and hepatic (HepG2) tumor cells were treated with varying concentrations of BCAA with or without fluorouracil (5-FU). The effect of BCAA on insulin-initiated proliferation was determined. Gene and protein expression was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting, respectively. RESULTS: BCAA supplementation had no significant effect on cell proliferation and did not show significant synergistic or antagonistic effects with 5-FU. However, BCAA significantly decreased insulin-initiated proliferation of human colon and hepatic cancer cell lines in vitro. BCAA supplementation caused a marked decrease in activated IGF-IR expression and significantly enhanced both mRNA and protein expression of LC3-II and BECN1 (BECLIN-1). CONCLUSION: BCAA could be a useful chemopreventive modality for cancer in hyperinsulinemic conditions. Copyright
BACKGROUND:Branched chain amino acid (BCAA) dietary supplementation inhibits activation of the insulin-like growth factor (IGF)/IGF-I receptor (IGF-IR) axis in diabetic animal models. However, the in vitro effect of BCAA on humancancer cell lines under hyper-insulinemic conditions remains unclear. MATERIALS AND METHODS: Colon (HCT-116) and hepatic (HepG2) tumor cells were treated with varying concentrations of BCAA with or without fluorouracil (5-FU). The effect of BCAA on insulin-initiated proliferation was determined. Gene and protein expression was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting, respectively. RESULTS:BCAA supplementation had no significant effect on cell proliferation and did not show significant synergistic or antagonistic effects with 5-FU. However, BCAA significantly decreased insulin-initiated proliferation of humancolon and hepatic cancer cell lines in vitro. BCAA supplementation caused a marked decrease in activated IGF-IR expression and significantly enhanced both mRNA and protein expression of LC3-II and BECN1 (BECLIN-1). CONCLUSION:BCAA could be a useful chemopreventive modality for cancer in hyperinsulinemic conditions. Copyright
Authors: Ryoko Katagiri; Mingyang Song; Xuehong Zhang; Dong Hoon Lee; Fred K Tabung; Charles S Fuchs; Jeffrey A Meyerhardt; Reiko Nishihara; Andrew T Chan; Amit D Joshi; Motoki Iwasaki; Shuji Ogino; Walter C Willett; Edward Giovannucci; Kana Wu Journal: Cancer Prev Res (Phila) Date: 2019-11-07
Authors: Jung Gil Park; Won Young Tak; Soo Young Park; Young Oh Kweon; Woo Jin Chung; Byoung Kuk Jang; Si Hyun Bae; Heon Ju Lee; Jae Young Jang; Ki Tae Suk; Myung Jin Oh; Jeong Heo; Hyun Young Woo; Se Young Jang; Yu Rim Lee; June Sung Lee; Do Young Kim; Seok Hyun Kim; Jeong Ill Suh; In Hee Kim; Min Kyu Kang; Won Kee Lee Journal: Nutrients Date: 2020-05-15 Impact factor: 5.717