Ryuji Suzuki1, Atsushi Aruga2, Hajime Kobayashi3, Masayuki Yamato3, Masakazu Yamamoto1. 1. Department of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan. 2. Department of Gastroenterological Surgery, Tokyo Women's Medical University, Tokyo, Japan Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan aruga.atsushi@twmu.ac.jp. 3. Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Abstract
AIM: Standard in vivo cancer models entail injecting single cancer cells, but this technique is not always successful. We developed a novel cancer cell sheet by using temperature-responsive polymer poly(N-isopropyl acryl amide)-coated plates, which allow controlled attachment and detachment of living cancer cells via simple temperature changes. MATERIALS AND METHODS: Four human cancer cell lines were used to make cell sheets. The cancer cell sheets were subcutaneously transplanted into nude mice and compared regarding their tumor-forming ability with the conventional cell suspension technique. RESULTS: Human cancer cell sheets were successfully transplanted into nude mice. The cancer cell sheets resulted in stable engraftment and showed a higher tumor volume determined by total flux with the IVIS® imaging system. CONCLUSION: Novel cancer cell sheets are useful tools to make in vivo cancer models in mice for the assessment of anticancer therapeutics. Copyright
AIM: Standard in vivo cancer models entail injecting single cancer cells, but this technique is not always successful. We developed a novel cancer cell sheet by using temperature-responsive polymerpoly(N-isopropyl acryl amide)-coated plates, which allow controlled attachment and detachment of living cancer cells via simple temperature changes. MATERIALS AND METHODS: Four humancancer cell lines were used to make cell sheets. The cancer cell sheets were subcutaneously transplanted into nude mice and compared regarding their tumor-forming ability with the conventional cell suspension technique. RESULTS:Humancancer cell sheets were successfully transplanted into nude mice. The cancer cell sheets resulted in stable engraftment and showed a higher tumor volume determined by total flux with the IVIS® imaging system. CONCLUSION: Novel cancer cell sheets are useful tools to make in vivo cancer models in mice for the assessment of anticancer therapeutics. Copyright