Corinne Lohri1, Claudia S Hutzli Schaltegger2, Maries VAN DEN Broek3, Roland H Wenger4, Curzio Ruegg5, Daniel Fink6, Mathias K Fehr7, Alexander Knuth8, Martin Zweifel9. 1. Buelach Hospital, Buelach, Switzerland. 2. Department of Gynaecology and Obstetrics, Maennedorf Hospital, Maennedorf, Switzerland. 3. Institute of Experimental Immunology, University of Zurich, Zurich, Switzerland. 4. Institute of Physiology, University of Zurich, Zurich, Switzerland Zurich Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland. 5. Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland. 6. Department of Gynaecology, University Hospital Zurich, Zurich, Switzerland. 7. Department of Gynaecology and Obstetrics, Frauenfeld General Hospital, Frauenfeld, Switzerland. 8. National Center for Cancer Care and Research, Hamad Medical Corporation, Doha, Qatar. 9. Department of Medical Oncology, Bern University Hospital, Bern, Switzerland martin.zweifel@insel.ch.
Abstract
BACKGROUND: Distinct populations of neutrophils have been identified based on the expression of intercellular adhesion molecule 1 (ICAM1, CD54) and chemokine receptor 1 (CXCR1, interleukin 8 receptor α). AIM: We analyzed the expression of vascular endothelial growth factor receptor 1 (VEGFR1), a physiological negative regulator of angiogenesis, on distinct populations of neutrophils from the blood of patients before and after adjuvant chemotherapy for breast cancer. MATERIALS AND METHODS: Neutrophil populations were distinguished as reverse transmigrated (ICAM1(high)/CXCR1(low)), naïve (ICAM1(low)/CXCR1(high)), or tissue-resident neutrophils (ICAM1(low)/CXCR1(low)), and their VEGFR1 expression quantified. RESULTS: Reverse transmigrated ICAM1(high)/CXCR1(low) neutrophilic granulocytes decreased significantly after chemotherapy and these were also the cells with highest mean fluorescence intensity for VEGFR1. CONCLUSION: Chemotherapy mainly reduces the number of reverse transmigrated long-lived ICAM1(high)/CXCR1(low) VEGFR1-expressing neutrophils. The decrease of antiangiogenic VEGFR1 may have a potential impact on tumour angiogenesis in patients undergoing adjuvant chemotherapy. Copyright
BACKGROUND: Distinct populations of neutrophils have been identified based on the expression of intercellular adhesion molecule 1 (ICAM1, CD54) and chemokine receptor 1 (CXCR1, interleukin 8 receptor α). AIM: We analyzed the expression of vascular endothelial growth factor receptor 1 (VEGFR1), a physiological negative regulator of angiogenesis, on distinct populations of neutrophils from the blood of patients before and after adjuvant chemotherapy for breast cancer. MATERIALS AND METHODS: Neutrophil populations were distinguished as reverse transmigrated (ICAM1(high)/CXCR1(low)), naïve (ICAM1(low)/CXCR1(high)), or tissue-resident neutrophils (ICAM1(low)/CXCR1(low)), and their VEGFR1 expression quantified. RESULTS: Reverse transmigrated ICAM1(high)/CXCR1(low) neutrophilic granulocytes decreased significantly after chemotherapy and these were also the cells with highest mean fluorescence intensity for VEGFR1. CONCLUSION: Chemotherapy mainly reduces the number of reverse transmigrated long-lived ICAM1(high)/CXCR1(low) VEGFR1-expressing neutrophils. The decrease of antiangiogenic VEGFR1 may have a potential impact on tumour angiogenesis in patients undergoing adjuvant chemotherapy. Copyright
Authors: Hannah L Wiesolek; Triet M Bui; Joseph J Lee; Prarthana Dalal; Ariel Finkielsztein; Ayush Batra; Edward B Thorp; Ronen Sumagin Journal: Am J Pathol Date: 2020-02-06 Impact factor: 4.307