Zizhang Ouyang1, Weiwei Cao1, Shaohua Zhu2, Xiaoping Liu1, Zhihua Zhong1, Xiangmao Lai1, Huirong Deng1, Sheng Jiang1, Yan Wang3. 1. Department of Pharmaceutical Sciences, Renmin Hospital of Qingyuan, The 5th Hospital Affiliated to Jinan University Qingyuan 511515, China. 2. School of Pharmaceutical Sciences, Sun Yat-Sen University Guangzhou 510006, China. 3. Renmin Hospital of Yichang, The First College of Clinical Medicine Affiliated to China Three Gorges University Yichang, China.
Abstract
OBJECTIVE: This study aims to explore the protective effect mechanism of 2-deoxy-D-glucose on nephrotoxicity of cyclosporin A in vivo. METHOD: Renal toxicity of SD rats model induced by CsA was established. Serum creatinine, blood urea nitrogen, urine NAG, GSH and MDA were determined and the histopathological changes of rat renal cortex were observed to explore the protective effects of 2-DG on CsA-induced nephrotoxicity. RESULTS: Serum creatinine, BUN and urinary NAG of rats were significantly changed in experimental groups. Pathological results showed that there was obvious renal tubular injury in model group, however, the renal injury was significantly reduced in pre-treated with 2-DG. CONCLUSIONS: 2-DG had obvious protective effect on nephrotoxicity especially with high dose. This protective effect could be related to the reduction of ROS induced by CsA. However, 2-DG had no effect on the expression of RIP3.
OBJECTIVE: This study aims to explore the protective effect mechanism of 2-deoxy-D-glucose on nephrotoxicity of cyclosporin A in vivo. METHOD:Renal toxicity of SD rats model induced by CsA was established. Serum creatinine, blood ureanitrogen, urine NAG, GSH and MDA were determined and the histopathological changes of rat renal cortex were observed to explore the protective effects of 2-DG on CsA-induced nephrotoxicity. RESULTS: Serum creatinine, BUN and urinary NAG of rats were significantly changed in experimental groups. Pathological results showed that there was obvious renal tubular injury in model group, however, the renal injury was significantly reduced in pre-treated with 2-DG. CONCLUSIONS:2-DG had obvious protective effect on nephrotoxicity especially with high dose. This protective effect could be related to the reduction of ROS induced by CsA. However, 2-DG had no effect on the expression of RIP3.
Entities:
Keywords:
2-deoxy-D-glucose; Cyclosporin A; GSH; MDA; RIP3; SD rats model
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