X Yao1, F Tang, M Yu, H Zhu, Z Chu, M Li, W Liu, J Hua, S Peng. 1. College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, 712100, China; Shaanxi Stem Cell Engineering and Technology Research Center, Northwest Agriculture and Forestry University, Yangling, 712100, China.
Abstract
OBJECTIVES: Nanos2, an RNA-binding protein, belongs to the Nanos gene-coding family and contains two CCHC zinc-finger motifs. In mouse, it plays a pivotal role in male germ cell development, and self-renewal of spermatogonial stem cells. However, little is known of its expression pattern and functions in dairy goat testis. MATERIALS AND METHODS: Immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to generate the expression profile of Nanos2 in dairy goat testis. Furthermore, its overexpression effects on male germline stem cells (mGSCs) were studied using qRT-PCR, immunofluorescence, dual-luciferase reporter assay and western blotting. RESULTS: Nanos2 is a conservative gene expressed widely in various tissues, especially in pancreas, and it displays higher expression in adult testes than in other age groups. Overexpression of Nanos2 significantly downregulated meiosis-related genes, including Stra8 and Scp3, which induced inhibition of meiosis. Results from dual-luciferase reporter assay and western blotting indicated that Nanos2 directly downregulated Stra8 in goat GmGSCs. CONCLUSIONS: Taken together, these results suggest that Nanos2 plays an important role in spermatogonia and that its overexpression restrained meiosis in the dairy goat.
OBJECTIVES:Nanos2, an RNA-binding protein, belongs to the Nanos gene-coding family and contains two CCHC zinc-finger motifs. In mouse, it plays a pivotal role in male germ cell development, and self-renewal of spermatogonial stem cells. However, little is known of its expression pattern and functions in dairy goat testis. MATERIALS AND METHODS: Immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) were used to generate the expression profile of Nanos2 in dairy goat testis. Furthermore, its overexpression effects on male germline stem cells (mGSCs) were studied using qRT-PCR, immunofluorescence, dual-luciferase reporter assay and western blotting. RESULTS:Nanos2 is a conservative gene expressed widely in various tissues, especially in pancreas, and it displays higher expression in adult testes than in other age groups. Overexpression of Nanos2 significantly downregulated meiosis-related genes, including Stra8 and Scp3, which induced inhibition of meiosis. Results from dual-luciferase reporter assay and western blotting indicated that Nanos2 directly downregulated Stra8 in goat GmGSCs. CONCLUSIONS: Taken together, these results suggest that Nanos2 plays an important role in spermatogonia and that its overexpression restrained meiosis in the dairy goat.
Authors: Ericka L Anderson; Andrew E Baltus; Hermien L Roepers-Gajadien; Terry J Hassold; Dirk G de Rooij; Ans M M van Pelt; David C Page Journal: Proc Natl Acad Sci U S A Date: 2008-09-17 Impact factor: 11.205
Authors: Yang Cui; Hailong Yan; Ke Wang; Han Xu; Xuelian Zhang; Haijing Zhu; Jinwang Liu; Lei Qu; Xianyong Lan; Chuanying Pan Journal: Front Genet Date: 2018-03-20 Impact factor: 4.599