| Literature DB >> 25193439 |
Victor S Pylro1, Luiz Fernando W Roesch2, Daniel K Morais3, Ian M Clark4, Penny R Hirsch4, Marcos R Tótola5.
Abstract
Progress in microbial ecology is confounded by problems when evaluating results from different sequencing methodologies. Contrary to existing expectations, here we demonstrate that the same biological conclusion is reached using different NGS technologies when stringent sequence quality filtering and accurate clustering algorithms are applied.Keywords: Alpha diversity; Amplicons; Beta diversity; Microbial community analysis; Next generation sequencing
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Year: 2014 PMID： 25193439 DOI： 10.1016/j.mimet.2014.08.018
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363