Seyma Sozen1, Cem Horozoglu1, Elif Sinem Bireller1, Zeynep Karaali2, Bedia Cakmakoglu3. 1. Department of Molecular Medicine, Institute for Experimental Medicine Research, Istanbul University, Istanbul, Turkey. 2. Department of Internal Medicine, Haseki Training and Research Hospital, Istanbul, Turkey. 3. Department of Molecular Medicine, Institute for Experimental Medicine Research, Istanbul University, Istanbul, Turkey bedia@istanbul.edu.tr.
Abstract
AIM: There are two different types of diabetes mellitus, type 1 and type 2, with still unclear molecular mechanisms. In the present study, we aimed to investigate the role of small ubiquitin-like modifier 4 (SUMO4) M55V and nuclear factor kappa B1 (NFKB1)-94del/ins in type-2 diabetes mellitus. MATERIALS AND METHODS: We analyzed SUMO4 M55V and NFKB1-94del/ins variants in 104 patients with type-2 diabetes and 124 healthy controls using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques. RESULTS: The number of SUMO4 M55V MM genotype and M allele carriers was significantly higher in patients compared to the control group; however, no efficiency results were found related to NFKB1-94del/ins polymorphism. CONCLUSION: It was found that SUMO4 M55V polymorphism and type-2 diabetes were significantly associated with a possible SUMO4 region to type-2 diabetes susceptibility. This preliminary study showed that the distribution of SUMO4 M55V and type-2 diabetes mellitus in Turkish patients may form the basis of future research.
AIM: There are two different types of diabetes mellitus, type 1 and type 2, with still unclear molecular mechanisms. In the present study, we aimed to investigate the role of small ubiquitin-like modifier 4 (SUMO4) M55V and nuclear factor kappa B1 (NFKB1)-94del/ins in type-2 diabetes mellitus. MATERIALS AND METHODS: We analyzed SUMO4M55V and NFKB1-94del/ins variants in 104 patients with type-2 diabetes and 124 healthy controls using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) techniques. RESULTS: The number of SUMO4M55V MM genotype and M allele carriers was significantly higher in patients compared to the control group; however, no efficiency results were found related to NFKB1-94del/ins polymorphism. CONCLUSION: It was found that SUMO4M55V polymorphism and type-2 diabetes were significantly associated with a possible SUMO4 region to type-2 diabetes susceptibility. This preliminary study showed that the distribution of SUMO4M55V and type-2 diabetes mellitus in Turkish patients may form the basis of future research.