Rene Garcia-Contreras1, Rogelio J Scougall-Vilchis2, Rosalia Contreras-Bulnes3, Yumiko Kanda4, Hiroshi Nakajima5, Hiroshi Sakagami6. 1. Dental and Advanced Studies Research Center, Faculty of Dentistry, Autonomous University State of Mexico, Toluca, Mexico Division of Pharmacology, Meikai University School of Dentistry, Sakado, Saitama, Japan sakagami@dent.meikai.ac.jp dentist.garcia@gmail.com. 2. Department of Orthodontics, Faculty of Dentistry, Autonomous University State of Mexico, Toluca, Mexico. 3. Department of Pediatric Dentistry, Faculty of Dentistry, Autonomous University State of Mexico, Toluca, Mexico. 4. Department of Electron Microscopy, Meikai University School of Dentistry, Sakado, Saitama, Japan. 5. Division of Dental Biomaterials Science, Meikai University School of Dentistry, Sakado, Saitama, Japan. 6. Division of Pharmacology, Meikai University School of Dentistry, Sakado, Saitama, Japan sakagami@dent.meikai.ac.jp dentist.garcia@gmail.com.
Abstract
BACKGROUND/AIM: Incorporation of nanoparticles (NPs) into the glass ionomer cements (GICs) is known to improve their mechanical and antibacterial properties. The present study aimed to investigate the possible cytotoxicity and pro-inflammation effect of three different powdered GICs (base, core build and restorative) prepared with and without titanium dioxide (TiO2) nanoparticles. MATERIALS AND METHODS: Each GIC was blended with TiO2 nanopowder, anatase phase, particle size <25 nm at 3% and 5% (w/w), and the GIC blocks of cements were prepared in a metal mold. The GICs/TiO2 nanoparticles cements were smashed up with a mortar and pestle to a fine powder, and then subjected to the sterilization by autoclaving. Human oral squamous cell carcinoma cell lines (HCS-2, HSC-3, HSC-4, Ca9-22) and human normal oral cells [gingival fibroblast (HGF), pulp (HPC) and periodontal ligament fibroblast (HPLF)] were incubated with different concentrations of GICs in the presence or absence of TiO2 nanoparticles, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Prostaglandin E2 was quantified by enzyme-linked immunosorbent assay (ELISA). Changes in fine cell structure were assessed by transmission electron microscopy. RESULTS: Cancer cells exhibited moderate cytotoxicity after 48 h of incubation, regardless of the type of GIC and the presence or absence of TiO2 NPs. GICs induced much lower cytotoxicity against normal cells, but induced prostaglandin E2 production, in a synergistic wanner with interleukin-1β. CONCLUSION: The present study shows acceptable to moderate biocompatibility of GICs impregnated with TiO2 nanoparticles, as well as its pro-inflammatory effects at higher concentrations.
BACKGROUND/AIM: Incorporation of nanoparticles (NPs) into the glass ionomer cements (GICs) is known to improve their mechanical and antibacterial properties. The present study aimed to investigate the possible cytotoxicity and pro-inflammation effect of three different powdered GICs (base, core build and restorative) prepared with and without titanium dioxide (TiO2) nanoparticles. MATERIALS AND METHODS: Each GIC was blended with TiO2 nanopowder, anatase phase, particle size <25 nm at 3% and 5% (w/w), and the GIC blocks of cements were prepared in a metal mold. The GICs/TiO2 nanoparticles cements were smashed up with a mortar and pestle to a fine powder, and then subjected to the sterilization by autoclaving. Humanoral squamous cell carcinoma cell lines (HCS-2, HSC-3, HSC-4, Ca9-22) and human normal oral cells [gingival fibroblast (HGF), pulp (HPC) and periodontal ligament fibroblast (HPLF)] were incubated with different concentrations of GICs in the presence or absence of TiO2 nanoparticles, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Prostaglandin E2 was quantified by enzyme-linked immunosorbent assay (ELISA). Changes in fine cell structure were assessed by transmission electron microscopy. RESULTS:Cancer cells exhibited moderate cytotoxicity after 48 h of incubation, regardless of the type of GIC and the presence or absence of TiO2 NPs. GICs induced much lower cytotoxicity against normal cells, but induced prostaglandin E2 production, in a synergistic wanner with interleukin-1β. CONCLUSION: The present study shows acceptable to moderate biocompatibility of GICs impregnated with TiO2 nanoparticles, as well as its pro-inflammatory effects at higher concentrations.
Authors: Shariq Najeeb; Zohaib Khurshid; Muhammad Sohail Zafar; Abdul Samad Khan; Sana Zohaib; Juan Manuel Nuñez Martí; Salvatore Sauro; Jukka Pekka Matinlinna; Ihtesham Ur Rehman Journal: Int J Mol Sci Date: 2016-07-14 Impact factor: 5.923