Literature DB >> 25187134

Enhanced production of recombinant galactose oxidase from Fusarium graminearum in E. coli.

Withu Choosri1, Regina Paukner, Petra Wührer, Dietmar Haltrich, Christian Leitner.   

Abstract

The gene gaoA encoding the copper-dependent enzyme galactose oxidase (GAO) from Fusarium graminearum PH-1 was cloned and successfully overexpressed in E. coli. Culture conditions for cultivations in shaken flasks were optimized, and optimal conditions were found to be double-strength LB medium, 0.5% lactose as inducer, and induction at the reduced temperature of 25°C. When using these cultivation conditions ~24 mg of active GAO could be produced in shaken flasks per litre medium. Addition of copper to the fermentation medium decreased the enzyme production significantly. The His-tagged recombinant enzyme could be purified conveniently with a single affinity chromatography step. The purified enzyme showed a single band on SDS-PAGE with an apparent molecular mass of 66 kDa and had kinetic properties similar to those of the fungal wild-type enzyme.

Entities:  

Year:  2010        PMID： 25187134      PMCID： PMC4410317          DOI： 10.1007/s11274-010-0585-2

Source DB:  PubMed          Journal:  World J Microbiol Biotechnol        ISSN： 0959-3993            Impact factor:   3.312


  18 in total

1.  Expression of recombinant galactose oxidase by Pichia pastoris.

Authors:  M M Whittaker; J W Whittaker
Journal:  Protein Expr Purif       Date:  2000-10       Impact factor: 1.650

2.  Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution.

Authors:  L Sun; I P Petrounia; M Yagasaki; G Bandara; F H Arnold
Journal:  Protein Eng       Date:  2001-09

3.  Integrated microbiosensors for clinical diagnosis.

Authors:  I Karube; J Kimura; K Yokoyama; E Tamiya
Journal:  Ann N Y Acad Sci       Date:  1990       Impact factor: 5.691

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Effect of culture conditions on the production of D-galactose oxidase by Dactylium dendroides.

Authors:  Z Markus; G Miller; G Avigad
Journal:  Appl Microbiol       Date:  1965-09

Review 6.  The radical chemistry of galactose oxidase.

Authors:  James W Whittaker
Journal:  Arch Biochem Biophys       Date:  2005-01-01       Impact factor: 4.013

7.  Light microscopic histochemical detection of sugar residues in secretory glycoproteins of rodent and human tracheal glands with lectin-horseradish peroxidase conjugates and the galactose oxidase-Schiff sequence.

Authors:  B A Schulte; S S Spicer
Journal:  J Histochem Cytochem       Date:  1983-03       Impact factor: 2.479

8.  Production, purification, and characterization of a novel galactose oxidase from Fusarium acuminatum.

Authors:  Dayane Alberton; Luciana Silva de Oliveira; Rosane Marina Peralta; Ione Parra Barbosa-Tessmann
Journal:  J Basic Microbiol       Date:  2007-06       Impact factor: 2.281

9.  Validation of the galactose oxidase-Schiff's reagent sequence for early detection and prognosis in human colorectal adenocarcinoma.

Authors:  J H Carter; J A Deddens; J L Pullman; B M Colligan; L O Whiteley; H W Carter
Journal:  Clin Cancer Res       Date:  1997-09       Impact factor: 12.531

10.  A new PCR approach for the identification of Fusarium graminearum.

Authors:  Gleison Ricardo de Biazio; Gabriela Guimarães Sousa Leite; Dauri José Tessmann; Ione Parra Barbosa-Tessmann
Journal:  Braz J Microbiol       Date:  2008-09-01       Impact factor: 2.476
View more
  6 in total

1.  A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family.

Authors:  Martina Andberg; Filip Mollerup; Kirsti Parikka; Sanna Koutaniemi; Harry Boer; Minna Juvonen; Emma Master; Maija Tenkanen; Kristiina Kruus
Journal:  Appl Environ Microbiol       Date:  2017-09-29       Impact factor: 4.792

2.  Production of Galactose Oxidase Inside the Fusarium fujikuroi Species Complex and Recombinant Expression and Characterization of the Galactose Oxidase GaoA Protein from Fusarium subglutinans.

Authors:  Carla Bertechini Faria; Fausto Fernandes de Castro; Damaris Batistão Martim; Camila Agnes Lumi Abe; Kelly Valério Prates; Marco Aurelio Schuler de Oliveira; Ione Parra Barbosa-Tessmann
Journal:  Mol Biotechnol       Date:  2019-09       Impact factor: 2.695

3.  Upscale production of a recombinant cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli.

Authors:  Yi-Nan Yang; Wen-Xin Shan; Pi-Wu Wang
Journal:  3 Biotech       Date:  2017-06-30       Impact factor: 2.406

4.  Expression, purification, and characterization of galactose oxidase of Fusarium sambucinum in E. coli.

Authors:  Regina Paukner; Petra Staudigl; Withu Choosri; Dietmar Haltrich; Christian Leitner
Journal:  Protein Expr Purif       Date:  2014-12-24       Impact factor: 1.650

5.  Galactose oxidase from Fusarium oxysporum--expression in E. coli and P. pastoris and biochemical characterization.

Authors:  Regina Paukner; Petra Staudigl; Withu Choosri; Christoph Sygmund; Petr Halada; Dietmar Haltrich; Christian Leitner
Journal:  PLoS One       Date:  2014-06-26       Impact factor: 3.240

6.  On the Mediated Electron Transfer of Immobilized Galactose Oxidase for Biotechnological Applications.

Authors:  Fangyuan Zhao; Ann Cathrin Brix; Anna Lielpetere; Wolfgang Schuhmann; Felipe Conzuelo
Journal:  Chemistry       Date:  2022-04-12       Impact factor: 5.020
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.