| Literature DB >> 25185697 |
Katarina Galic Jerman, Borut Kobal, Marina Jakimovska, Ivan Verdenik, Katarina Cerne1.
Abstract
BACKGROUND: Determination of the tumor marker concentration in peritoneal fluid (PF) may help to assess its potential to detect small concentration changes between benign ovarian pathology and early stage ovarian cancer. Peritoneal washing, which can also be obtained when PF is absent, is already included in the International Federation of Gynecology and Obstetrics (FIGO) staging classification for ovarian cancer but sampling has not yet been standardized. Since our aim was to evaluate the relationship between marker concentration in PF and washing, standardization of the sampling protocol was a prerequisite to ensure reliable results.Entities:
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Year: 2014 PMID: 25185697 PMCID: PMC4167500 DOI: 10.1186/1477-7819-12-278
Source DB: PubMed Journal: World J Surg Oncol ISSN: 1477-7819 Impact factor: 2.754
Patient characteristics
| Parameters | |
|---|---|
| Number of patients | 33 |
| age (average ± SEM) | 43 ± 1.82 years |
| range | 21-69 years |
|
| |
| benign ovarian cyst | 8 (24%) |
| myoma of the uterus | 16 (48%) |
| pelvic pain, sterilization | 7 (24%) |
| preventive adnexectomy | 2 (6%) |
|
| |
| reproductive | 23 (70%) |
| perimenopausal | 6 (18%) |
| Postmenopausal | 4 (12%) |
|
| |
| luteal | 10 (30%) |
| follicular | 7 (21%) |
| not applicable | 16 (48%) |
|
| |
| present, number of patients (%) | 26 (79%) |
| absent, number of patients (%) | 7 (21%) |
| volume (mean ± SEM) | 6.03 ± 1.22 ml |
|
| |
| intrauterine device | 4 (12%) |
| combined hormone therapy | 3 (9%) |
Concentrations of sOPN, sCD44-v6 and sVCAM-1 (average ± SEM) in peritoneal fluid and in washings performed with 0.9% NaCl solution
| Sample (number of patients) | sOPN | sCD44-v6 | sVCAM-1 |
|---|---|---|---|
|
| |||
|
| |||
| Peritoneal fluid (26) | 132.1 ± 22.9 | 45.3 ± 3.4 | 438.8 ± 11.7 |
| range | 21.4 - 483.1 | 19.3 - 89.3 | 311.8 - 588.43 |
|
| |||
| Washing 1A (26) | 20.0 ± 6.5 | 4.6 ± 0.5 | 108.2 ± 14.7 |
| Washing 2A (20) | 7.2 ± 2.7 | 2.3 ± 0.4 | 34.4 ± 6.7 |
| Washing 3A (20) | 6.0 ± 3.0 | 1.8 ± 0.4 | 28.3 ± 6.7 |
|
| |||
| Washing 1B (7) | 6.7 ± 1.7 | 5.5 ± 1.3 | 46.2 ± 11.6 |
| Washing 2B (7) | 3.0 ± 1.8 | 2.6 ± 0.9 | 20.7 ± 6.7 |
| Washing 3B (7) | 2.2 ± 0.5 | 2.1 ± 1.0 | 22.1 ± 8.5 |
|
| |||
| Peritoneal fluid : washing 1A | 6.6 | 9.8 | 4.1 |
Figure 1Correlation of sOPN, sCD44-v6 and sVCAM-1 concentrations between first and second washings, as well as between second and third washings. Three washings were performed consecutively in patients of group A with 20 ml, 20 ml and 10 ml of 0.9% NaCl solution.
Figure 2Comparison of absolute quantity (ng) of sOPN, sCD44-v6 and sVCAM-1 among three consecutively performed washings, as well as between group A (black bars) and group B (grey bars). In comparison to the first, the absolute quantity of all three tumor markers significantly decreased in the second and third washings within both groups. There was no significant difference in absolute quantity of sOPN and sVCAM-1 between groups A and B in any of the three washings. However, there was a significant difference in absolute quantity of sCD44-v6 between groups A and B in all three washings. Washings were performed consecutively in patients of group A with a smaller volume of solution (1A: 20 ml, 2A: 20 ml and 3A: 10 ml) and in patients of group B with a larger volume of solution (1B: 50 ml, 2B: 50 ml and 3B: 20 ml). Tumor marker absolute quantity was calculated by multiplying tumor marker concentration (ng/ml) with the volume of aspirated washing (ml). *P <0.05, **P <0.01, ***P <0.001.
Figure 3Correlation of sOPN, sCD44-v6 and sVCAM-1 concentrations between peritoneal fluid and the first washing in patients of group A. Washing was performed with 20 ml of 0.9% NaCl solution.