Kanokwan Kulprachakarn1, Nittaya Chansiw1, Kanjana Pangjit2, Chada Phisalaphong3, Suthat Fucharoen4, Robert C Hider5, Sineenart Santitherakul6, Somdet Srichairatanakool1. 1. Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. 2. College of Medicine and Public Health, Ubon Ratchathani University, Ubon Ratchathani, Thailand. 3. Institute of Research and Development, Government Pharmaceuticals Organization, Ministry of Public Health, Thailand. 4. Thalassemia Research Center, Institute of Molecular Biosciences, Mahidol University Salaya Campus, Nakornprathom, Thailand. 5. Institute of Pharmaceutical Science, King's College London, Franklin-Wilkins Building, London, United Kingdom. 6. Medical Science Research Equipment Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Abstract
OBJECTIVE: To evaluate the iron-chelating properties and free-radical scavenging activities of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) treatment in chronic iron-loaded β-thalassemic (BKO) mice. METHODS: The BKO mice were fed with a ferrocene-rich diet and were orally administered with CM1 [50 mg/(kg.day)] for 6 months. Blood levels of non-transferrin bound iron, labile plasma iron, ferritin (Ft) and malondialdehyde were determined. RESULTS: The BKO mice were fed with an iron diet for 8 months which resulted in iron overload. Interestingly, the mice showed a decrease in the non-transferrin bound iron, labile plasma iron and malondialdehyde levels, but not the Ft levels after continuous CM1 treatment. CONCLUSIONS: CM1 could be an effective oral iron chelator that can reduce iron overload and lipid peroxidation in chronic iron overload β-thalassemic mice.
OBJECTIVE: To evaluate the iron-chelating properties and free-radical scavenging activities of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) treatment in chronic iron-loaded β-thalassemic (BKO) mice. METHODS: The BKO mice were fed with a ferrocene-rich diet and were orally administered with CM1 [50 mg/(kg.day)] for 6 months. Blood levels of non-transferrin bound iron, labile plasma iron, ferritin (Ft) and malondialdehyde were determined. RESULTS: The BKO mice were fed with an iron diet for 8 months which resulted in iron overload. Interestingly, the mice showed a decrease in the non-transferrin bound iron, labile plasma iron and malondialdehyde levels, but not the Ft levels after continuous CM1 treatment. CONCLUSIONS: CM1 could be an effective oral iron chelator that can reduce iron overload and lipid peroxidation in chronic iron overload β-thalassemic mice.
Entities:
Keywords:
Iron chelator; Iron overload; Iron-chelating; Lipid peroxidation; Non-transferrin bound iron; β-thalassemia
Authors: D Grotto; L D Santa Maria; S Boeira; J Valentini; M F Charão; A M Moro; P C Nascimento; V J Pomblum; S C Garcia Journal: J Pharm Biomed Anal Date: 2006-09-01 Impact factor: 3.935