Hakan Söyüt1, Elif Duygu Kaya2, Sükrü Beydemir3. 1. Department of Primary Education, Faculty of Education, Bayburt University, 69000 Bayburt, Turkey. 2. Department of Food Engineering, Faculty of Engineering, Iğdır University, 76000 Iğdır, Turkey. 3. Department of Chemistry, Faculty of Science, Atatürk University, 25240 Erzurum, Turkey.
Abstract
OBJECTIVE: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1). METHODS: hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. RESULTS: The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive. CONCLUSIONS: Our results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.
OBJECTIVE: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1). METHODS:hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. RESULTS: The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive. CONCLUSIONS: Our results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydratepiperacillin sodium cefoperazone sodium in vitro.