OBJECTIVE: To study the genetic diversity at the msp-1, msp-2, and glurp genes of Plasmodium falciparum (P. falciparum) isolates from 3 endemic areas in Thailand: Tak, Kanchanaburi and Ranong provinces. METHODS: A total of 144 P. falciparum isolates collected prior to treatment during January, 2012 to June, 2013 were genotyped. DNA was extracted; allele frequency and diversity of msp-1, msp-2, and glurp genes were investigated by nested polymerase chain reaction. RESULTS: P. falciparum isolates in this study had high rate of multiple genotypes infection (96.5%) with an overall mean multiplicity of infection of 3.21. The distribution of allelic families of msp-1 was significantly different among isolates from Tak, Kanchanaburi, and Ranong but not for the msp-2. K1 and MAD20 were the predominant allelic families at the msp-1 gene, whereas alleles belonging to 3D7 were more frequent at the msp-2 gene. The glurp gene had the least diverse alleles. Population structure of P. falciparum isolates from Tak and Ranong was quite similar as revealed by the presence of similar proportions of MAD20 and K1 alleles at msp-1 loci, 3D7 and FC27 alleles at msp-2 loci as well as comparable mean MOI. Isolates from Kanchanaburi had different structures; the most prevalent alleles were K1 and RO33. CONCLUSIONS: The present study shows that P. falciparum isolates from Tak and Ranong provinces had similar allelic pattern of msp-1 and msp-2 and diversity but different from Kanchanaburi isolates. These allelic variant profiles are valuable baseline data for future epidemiological study of malaria transmission and for continued monitoring of polymorphisms associated with antimalarial drug resistance in these areas.
OBJECTIVE: To study the genetic diversity at the msp-1, msp-2, and glurp genes of Plasmodium falciparum (P. falciparum) isolates from 3 endemic areas in Thailand: Tak, Kanchanaburi and Ranong provinces. METHODS: A total of 144 P. falciparum isolates collected prior to treatment during January, 2012 to June, 2013 were genotyped. DNA was extracted; allele frequency and diversity of msp-1, msp-2, and glurp genes were investigated by nested polymerase chain reaction. RESULTS:P. falciparum isolates in this study had high rate of multiple genotypes infection (96.5%) with an overall mean multiplicity of infection of 3.21. The distribution of allelic families of msp-1 was significantly different among isolates from Tak, Kanchanaburi, and Ranong but not for the msp-2. K1 and MAD20 were the predominant allelic families at the msp-1 gene, whereas alleles belonging to 3D7 were more frequent at the msp-2 gene. The glurp gene had the least diverse alleles. Population structure of P. falciparum isolates from Tak and Ranong was quite similar as revealed by the presence of similar proportions of MAD20 and K1 alleles at msp-1 loci, 3D7 and FC27 alleles at msp-2 loci as well as comparable mean MOI. Isolates from Kanchanaburi had different structures; the most prevalent alleles were K1 and RO33. CONCLUSIONS: The present study shows that P. falciparum isolates from Tak and Ranong provinces had similar allelic pattern of msp-1 and msp-2 and diversity but different from Kanchanaburi isolates. These allelic variant profiles are valuable baseline data for future epidemiological study of malaria transmission and for continued monitoring of polymorphisms associated with antimalarial drug resistance in these areas.
Authors: Adithya Cattamanchi; Daniel Kyabayinze; Alan Hubbard; Philip J Rosenthal; Grant Dorsey Journal: Am J Trop Med Hyg Date: 2003-02 Impact factor: 2.345
Authors: G Snounou; X Zhu; N Siripoon; W Jarra; S Thaithong; K N Brown; S Viriyakosol Journal: Trans R Soc Trop Med Hyg Date: 1999 Jul-Aug Impact factor: 2.184
Authors: Lilian Rose Pratt-Riccio; Daiana de Souza Perce-da-Silva; Josué da Costa Lima-Junior; Michael Theisen; Fátima Santos; Cláudio Tadeu Daniel-Ribeiro; Joseli de Oliveira-Ferreira; Dalma Maria Banic Journal: Mem Inst Oswaldo Cruz Date: 2013-06 Impact factor: 2.743
Authors: Hema Joshi; Neena Valecha; Anju Verma; Asha Kaul; Prashant K Mallick; Sneh Shalini; Surendra K Prajapati; Surya K Sharma; Vas Dev; Sukla Biswas; Nutan Nanda; M S Malhotra; Sarala K Subbarao; Aditya P Dash Journal: Malar J Date: 2007-05-21 Impact factor: 2.979
Authors: Mohammad Shafiul Alam; Rubayet Elahi; Abu Naser Mohon; Hasan Mohammad Al-Amin; Mohammad Golam Kibria; Wasif A Khan; Hamida Khanum; Rashidul Haque Journal: Am J Trop Med Hyg Date: 2016-05-02 Impact factor: 2.345
Authors: Mohd Ridzuan Mohd Abd Razak; Umi Rubiah Sastu; Nor Azrina Norahmad; Abass Abdul-Karim; Amirrudin Muhammad; Prem Kumar Muniandy; Jenarun Jelip; Christina Rundi; Mallika Imwong; Rose Nani Mudin; Noor Rain Abdullah Journal: PLoS One Date: 2016-03-29 Impact factor: 3.240