Tobias Fretwurst1, Alexandra Spanou2, Katja Nelson3, Martin Wein4, Thorsten Steinberg4, Andres Stricker3. 1. Department of Oral and Craniomaxillofacial Surgery, University Medical Center Freiburg, Hugstetter Straße 55, D-79106, Freiburg, Germany. Electronic address: Tobias.fretwurst@uniklinik-freiburg.de. 2. Private Clinic, Athens, Greece. 3. Department of Oral and Craniomaxillofacial Surgery, University Medical Center Freiburg, Hugstetter Straße 55, D-79106, Freiburg, Germany. 4. Department of Oral Biotechnology, University Medical Center Freiburg, Hugstetter Straße 55, D-79106, Freiburg, Germany.
Abstract
OBJECTIVES: Allograft material for alveolar ridge reconstruction is quite promising and appears to be as equally successful as bone autograft material. The aim of the present study was to compare four different allogeneic bone grafts in terms of their histologic structure and DNA content before grafting. STUDY DESIGN: Four allograft specimens from different suppliers were analyzed histologically, and the DNA content was analyzed before clinical use of the allografts. RESULTS: Organic tissue remnants were detected in all of the evaluated samples. In the present samples adipocytes, fibroblasts, osteocytes, and chondrocytes were identified and DNA isolation and purification was possible. CONCLUSION: Demineralized freeze-dried allogeneic bone transplants can stimulate new bone formation and are a viable alternative to bone autograft material. However, the well-tolerated use of allograft material in regard to our findings should be further investigated.
OBJECTIVES: Allograft material for alveolar ridge reconstruction is quite promising and appears to be as equally successful as bone autograft material. The aim of the present study was to compare four different allogeneic bone grafts in terms of their histologic structure and DNA content before grafting. STUDY DESIGN: Four allograft specimens from different suppliers were analyzed histologically, and the DNA content was analyzed before clinical use of the allografts. RESULTS: Organic tissue remnants were detected in all of the evaluated samples. In the present samples adipocytes, fibroblasts, osteocytes, and chondrocytes were identified and DNA isolation and purification was possible. CONCLUSION: Demineralized freeze-dried allogeneic bone transplants can stimulate new bone formation and are a viable alternative to bone autograft material. However, the well-tolerated use of allograft material in regard to our findings should be further investigated.
Authors: Jonas Lorenz; Alica Kubesch; Sarah Al-Maawi; Frank Schwarz; Robert A Sader; Markus Schlee; Shahram Ghanaati Journal: Clin Oral Investig Date: 2018-03-09 Impact factor: 3.573
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Authors: Marco Cicciù; Gabriele Cervino; Alan Scott Herford; Fausto Famà; Ennio Bramanti; Luca Fiorillo; Floriana Lauritano; Sergio Sambataro; Giuseppe Troiano; Luigi Laino Journal: Mar Drugs Date: 2018-01-13 Impact factor: 5.118