Lotfi Ghribia1, Hatem Ghouilaa1, Amel Omrib2, Malek Besbesb2, Hichem Ben Janneta1. 1. Laboratoire de Chimie hétérocyclique, Produits Naturels et Réactivité. Equipe: Chimie Médicinale et Produits Naturels. Faculté des Sciences de Monastir, Université de Monastir 5019, Monastir, Tunisie. 2. Laboratoire des Maladies Transmissibles et des Substances Biologiquement Actives, Faculté de Pharmacie, 5000 Monastir, Tunisie.
Abstract
OBJECTIVE: To investigate the antioxidant potential and anti-acetycholinesterase activity of compounds and extracts from Acacia cyanophylla (A. cyanophylla). METHODS: Three polyphenolic compounds were isolated from ethyl acetate extract of A. cyanophylla flowers. They have been identified as isosalipurposide 1, quercetin 2 and naringenin 3. Their structures were elucidated by extensive spectroscopic methods including 1D and 2D NMR experiments as well as ES-MS. The prepared extracts and the isolated compounds 1-3 were tested for their antioxidant activity using 1'-1'-diphenylpicrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging assays and reducing power. They have been also investigated for inhibitory effect against acetylcholinesterase using the microplate assay. RESULTS: In the DPPH test, the EtOAc extract of flowers exhibited the highest antioxidant effect (67.26 µg/mL). Isosalipurposide 1 showed a significant antiradical power against DPPH (81.9 µg/mL). All extracts showed a dose-dependent acetylcholinesterase inhibition. In terms of the IC50 value, the butanolic extract (16.03 µg/mL) was the most potent sample. Isosalipurposide 1 was found to be active against AChE with an IC50 value of 52.04 µg/mL. CONCLUSIONS: The results demonstrated the important antioxidant and anti-acetylcholinesterase activity of pure compounds and extracts from A. cyanophylla.
OBJECTIVE: To investigate the antioxidant potential and anti-acetycholinesterase activity of compounds and extracts from Acacia cyanophylla (A. cyanophylla). METHODS: Three polyphenolic compounds were isolated from ethyl acetate extract of A. cyanophylla flowers. They have been identified as isosalipurposide 1, quercetin 2 and naringenin 3. Their structures were elucidated by extensive spectroscopic methods including 1D and 2D NMR experiments as well as ES-MS. The prepared extracts and the isolated compounds 1-3 were tested for their antioxidant activity using 1'-1'-diphenylpicrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging assays and reducing power. They have been also investigated for inhibitory effect against acetylcholinesterase using the microplate assay. RESULTS: In the DPPH test, the EtOAc extract of flowers exhibited the highest antioxidant effect (67.26 µg/mL). Isosalipurposide 1 showed a significant antiradical power against DPPH (81.9 µg/mL). All extracts showed a dose-dependent acetylcholinesterase inhibition. In terms of the IC50 value, the butanolic extract (16.03 µg/mL) was the most potent sample. Isosalipurposide 1 was found to be active against AChE with an IC50 value of 52.04 µg/mL. CONCLUSIONS: The results demonstrated the important antioxidant and anti-acetylcholinesterase activity of pure compounds and extracts from A. cyanophylla.
Authors: Dawood Shah; Arshad Iqbal; Fahad S Alshehri; Aman Ullah; Gowhar Ali; Tahir Muhammad; Rahim Ullah; Robert D E Sewell; Yusuf S Althobaiti Journal: J Inflamm Res Date: 2022-08-22
Authors: Carlos R Polaquini; Guilherme S Torrezan; Vanessa R Santos; Ana C Nazaré; Débora L Campos; Laíza A Almeida; Isabel C Silva; Henrique Ferreira; Fernando R Pavan; Cristiane Duque; Luis O Regasini Journal: Molecules Date: 2017-10-10 Impact factor: 4.411