| Literature DB >> 25182766 |
Cheryl S Watson1, Yow-Jiun Jeng, Nataliya N Bulayeva, Celeste C Finnerty, Luke Y Koong, Dragoslaca Zivadinovic, Rebecca A Alyea, Terumi Midoro-Horiuti, Randall M Goldblum, Noelle C Anastasio, Kathryn A Cunningham, Patricia K Seitz, Thressa D Smith.
Abstract
We developed fixed-cell multi-well plate immunoassays that increase the throughput and ease of quantification for questions formerly assessed by immunoblot scanning. The assays make use of the now abundant antibodies designed to recognize receptor subtypes and posttranslationally modified signaling proteins. By optimizing permeabilization and fixation conditions, mainly based on specific cell types, the assay can be adapted to the study of many different antigens of importance to hormonal and neurotransmitter signaling scenarios.Entities:
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Year: 2014 PMID： 25182766 PMCID： PMC9159966 DOI： 10.1007/978-1-4939-1346-6_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745