OBJECTIVE: To evaluate the 15-lipoxygenase inhibitory activity of the methanol leaf extracts of Commelina benghalensis, Tradescantia fluminensis (T. fluminensis) and Tradescantia zebrina. METHOD: The inhibitory activity was evaluated using a spectrophotometric assay by observing the increase in absorbance at 234 nm due to the formation of the product 13-hydroperoxyoctadecadienoic acid. The extracts were also tested for the presence of terpenoids, saponins, tannins, flavonoids, steroids, phenolic compounds, alkaloids and cardiac glycosides. RESULTS: All the extracts inhibited the action of 15-lipoxygenase at a concentration of 0.2 µg/mL. T. fluminensis and Tradescantia zebrina exhibited higher than 50% inhibition with T. fluminensis at 87.2%. T. fluminensis was partitioned with ethyl acetate and hexane and their IC50 values were determined at 8.72 µg/mL and 98.04 µg/mL, respectively. CONCLUSIONS: T. fluminensis is a potentially good source of 15-lipoxygenase inhibitors.
OBJECTIVE: To evaluate the 15-lipoxygenase inhibitory activity of the methanol leaf extracts of Commelina benghalensis, Tradescantia fluminensis (T. fluminensis) and Tradescantia zebrina. METHOD: The inhibitory activity was evaluated using a spectrophotometric assay by observing the increase in absorbance at 234 nm due to the formation of the product 13-hydroperoxyoctadecadienoic acid. The extracts were also tested for the presence of terpenoids, saponins, tannins, flavonoids, steroids, phenolic compounds, alkaloids and cardiac glycosides. RESULTS: All the extracts inhibited the action of 15-lipoxygenase at a concentration of 0.2 µg/mL. T. fluminensis and Tradescantia zebrina exhibited higher than 50% inhibition with T. fluminensis at 87.2%. T. fluminensis was partitioned with ethyl acetate and hexane and their IC50 values were determined at 8.72 µg/mL and 98.04 µg/mL, respectively. CONCLUSIONS:T. fluminensis is a potentially good source of 15-lipoxygenase inhibitors.
Authors: Khehyong Ngu; David S Weinstein; Wen Liu; Charles Langevine; Donald W Combs; Shaobin Zhuang; Xing Chen; Cort S Madsen; Timothy W Harper; Saleem Ahmad; Jeffrey A Robl Journal: Bioorg Med Chem Lett Date: 2011-06-06 Impact factor: 2.823
Authors: Louis A Rosenthal; Pedro C Avila; Peter W Heymann; Richard J Martin; E Kathryn Miller; Nikolaos G Papadopoulos; R Stokes Peebles; James E Gern Journal: J Allergy Clin Immunol Date: 2010-06 Impact factor: 10.793