BACKGROUND: Lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) of type 2 diabetes patients produce more interleukin (IL)-12 under glucose treatment. The aim of this study was to determine whether increased IL-12 response in hyperglycemic LPS-stimulated PBMCs is due to increased gene expression or osmolarity. METHODS: LPS-stimulated PBMCs of 13 type 2 diabetes patients and 8 healthy controls were used for culture in the presence or absence of glucose or mannitol for 24 h. The IL-12 gene expressions of PBMCs and IL-12 protein levels in supernatants were evaluated. RESULTS: After 24 h, the stimulated PBMCs of diabetes patients expressed more IL-12 mRNA and produced more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment. Stimulated PBMCs of controls did not express more IL-12 mRNA and produce more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment. CONCLUSIONS: Glucose increases the IL-12 production in stimulated PBMCs of diabetes patients through increased IL-12 gene expression.
BACKGROUND:Lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) of type 2 diabetespatients produce more interleukin (IL)-12 under glucose treatment. The aim of this study was to determine whether increased IL-12 response in hyperglycemic LPS-stimulated PBMCs is due to increased gene expression or osmolarity. METHODS:LPS-stimulated PBMCs of 13 type 2 diabetespatients and 8 healthy controls were used for culture in the presence or absence of glucose or mannitol for 24 h. The IL-12 gene expressions of PBMCs and IL-12 protein levels in supernatants were evaluated. RESULTS: After 24 h, the stimulated PBMCs of diabetespatients expressed more IL-12 mRNA and produced more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment. Stimulated PBMCs of controls did not express more IL-12 mRNA and produce more IL-12 protein following glucose treatment than those without glucose treatment and with mannitol treatment. CONCLUSIONS:Glucose increases the IL-12 production in stimulated PBMCs of diabetespatients through increased IL-12 gene expression.
Authors: Maha Ali; Vishal Mali; Samuel Haddox; Soad M AbdelGhany; Sahar E M El-Deek; Atif Abulfadl; Khalid Matrougui; Souad Belmadani Journal: Am J Pathol Date: 2017-08-22 Impact factor: 4.307