Qin Ren-an1, Lin Juan2, Li Chuyuan1, Fu Wenjuan2, Huang Chunyan2, Yu Xuemei2, Huang Lin1, Nie Hong3. 1. Guangzhou General Medicine & Pharmaceutical Research Institute, Guangzhou, PR China. 2. Jinan University College of Pharmacy, Guangzhou 510632, PR China. 3. Jinan University College of Pharmacy, Guangzhou 510632, PR China. Electronic address: hongnie1970@163.com.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Compound Danshen Tablet (CDT), an herbal preparation consisting of Salvia miltiorrhiza (Radix and rhizome of Salvia miltiorrhiza Bge.), Notoginseng (Radix and rhizome of Panax notoginseng (Burk.) F. H. Chen) and Borneolum Syntheticum, is widely used to improve coronary heart disease, cardiac angina and atherosclerosis in clinic in Asia and Pacific Ocean area, especially in China. AIM: The study is to research the protective mechanisms of Compound Danshen Tablet (CDT) against myocardial ischemia/reperfusion (MI/R) injury via the Akt-eNOS signaling Pathway in rats. MATERIALS AND METHODS: Rats were randomized into 7 groups: Sham group; Model group; Low-Dose CDT group (0.315 g/kg/d, i.g); Middle-Dose CDT group (0.63 g/kg/d, i.g); High-Dose CDT group (1.26 g/kg/d, i.g); Compound Danshen Dripping Pills (CDDP) group (0.0945 g/kg/d, i.g); Sulfotanshinone Sodium Injection (Tan II A) group (5 mg/kg/d, i.m). After the administration, the hearts of the rats were subjected to 30 min of coronary artery occlusion and 2h of reperfusion except the Sham group rats. RESULTS: CDT significantly decreased infarct size, apoptosis, caspase-3 protein expression, MDA level in myocardial tissues, the activities of serum CK, LDH and cTnI; on the contrary, it increased p-Akt, p-eNOS, bcl-2 protein expression, the activities of SOD and tissue LDH, and the level of NO. CONCLUSIONS: CDT can protect cardiomyocytes against MI/R injury and inhibits apoptosis in rats by activating Akt-eNOS signaling pathway.
ETHNOPHARMACOLOGICAL RELEVANCE: Compound Danshen Tablet (CDT), an herbal preparation consisting of Salvia miltiorrhiza (Radix and rhizome of Salvia miltiorrhiza Bge.), Notoginseng (Radix and rhizome of Panax notoginseng (Burk.) F. H. Chen) and Borneolum Syntheticum, is widely used to improve coronary heart disease, cardiac angina and atherosclerosis in clinic in Asia and Pacific Ocean area, especially in China. AIM: The study is to research the protective mechanisms of Compound Danshen Tablet (CDT) against myocardial ischemia/reperfusion (MI/R) injury via the Akt-eNOS signaling Pathway in rats. MATERIALS AND METHODS:Rats were randomized into 7 groups: Sham group; Model group; Low-Dose CDT group (0.315 g/kg/d, i.g); Middle-Dose CDT group (0.63 g/kg/d, i.g); High-Dose CDT group (1.26 g/kg/d, i.g); Compound Danshen Dripping Pills (CDDP) group (0.0945 g/kg/d, i.g); Sulfotanshinone Sodium Injection (Tan II A) group (5 mg/kg/d, i.m). After the administration, the hearts of the rats were subjected to 30 min of coronary artery occlusion and 2h of reperfusion except the Sham group rats. RESULTS:CDT significantly decreased infarct size, apoptosis, caspase-3 protein expression, MDA level in myocardial tissues, the activities of serum CK, LDH and cTnI; on the contrary, it increased p-Akt, p-eNOS, bcl-2 protein expression, the activities of SOD and tissue LDH, and the level of NO. CONCLUSIONS:CDT can protect cardiomyocytes against MI/R injury and inhibits apoptosis in rats by activating Akt-eNOS signaling pathway.