| Literature DB >> 25173166 |
Tomoyuki Yamaguchi1, Sanae Hamanaka, Hiromitsu Nakauchi.
Abstract
This chapter describes a newly developed method for generating and maintaining rat induced pluripotent stem cells (riPSCs). We first provide a detailed protocol for the generation of lentiviral vector carrying three reprogramming factors to produce high-quality riPSCs. This technique allows reprogramming of rat somatic cells to ground state with germ-line competence. Subsequently, we elaborate a detailed protocol for the generation of riPSCs from rat embryonic fibroblast (REF). Finally, the protocols for the optimal culture conditions of riPSCs and preparation of frozen stock are described. We also outline the advantages of generating riPSCs.Entities:
Mesh：
Year: 2014 PMID： 25173166 DOI： 10.1007/978-1-4939-1435-7_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745