Consequences of the matrix effect on recovery of dinotefuran and its metabolites in green tea during tandem mass spectrometry analysis.

Determining the residues of dinotefuran and its metabolites (MNG, UF, and DN) is highly problematic because of their polar characteristics. Additionally, tea contains many compounds that can interfere with residue analysis. Thus, the aim of the present study was to refine the extraction method that assures good recoveries for dinotefuran and its metabolites and removes most of the matrix components in green tea using liquid chromatography-tandem mass spectrometry (LC/MS/MS). We attempted to increase the extraction efficiency of the QuEChERS method by selecting the appropriate solvents among ethyl acetate, acetone, isopropanol, 25% methanol in acetonitrile, and methanol. We found that methanol was the best extraction solvent for dinotefuran and its polar metabolites in dry green tea samples; however, due to a limitation of an appropriate partitioning salt, acetonitrile was used as the extraction solvent. Matrix enhancement and suppression effects were observed for all analytes, which made the recovery rates variable. DN recovery was <70% when compared with matrix-matched calibration, whereas it was within the acceptable range (70-120%) when compared with solvent calibration. The opposite was observed for MNG and dinotefuran due to a matrix suppression effect. UF recovery was consistent in both matrix-matched and solvent calibrations despite having little suppressive effect. The method was successfully applied and dinotefuran and its metabolite residues were found in field-incurred green tea samples. The current findings suggest that using methanol as an appropriate QuEChERS solvent for problematic polar pesticides and investigating a suitable partitioning salt would considerably strengthen the practical impact of such data.