BACKGROUND: The cytokinesis-block micronucleus (CBMN) assay is a standard cytogenetic tool employed to evaluate chromosomal damage subsequent to pesticide exposure. OBJECTIVES: To evaluate the pooled levels of total micronuclei (MN) and binucleated cells with micronuclei (MNC) in 1000 binucleated lymphocytes among population occupationally exposed to pesticides and further determine the more sensitive biomarker of CBMN. MATERIALS AND METHODS: A meta-analysis on the pooled levels of MN and MNC in binucleated lymphocytes among occupationally pesticide-exposed populations was conducted using STATA 10.0 software and Review Manager 5.0.24 in this study. RESULTS: We found significant differences in frequencies of MN and MNC in 1000 binucleated lymphocytes between pesticide-exposed groups and controls, and the summary estimates of weighted mean difference were 6.82 [95% confidence interval (95% CI): 4.86-8.78] and 5.08 (95% CI: 2.93-7.23), respectively. However, when we conducted sensitivity analyses further, only the MN remained statistically different, but not the MNC, the summary estimates of weight mean difference were 2.86 (95% CI: 2.51-3.21) and 0.50 (95% CI: -0.16-1.17), respectively. We also observed pesticide-exposed subjects had significantly higher MN frequencies than controls among smokers and nonsmokers, male and female populations, and American, Asian and European countries in stratified analyses. CONCLUSIONS: The frequency of MN in peripheral blood lymphocytes might be a more sensitive indicator of early genetic effects than MNC using the CBMN assay for occupationally pesticide- exposed populations.
BACKGROUND: The cytokinesis-block micronucleus (CBMN) assay is a standard cytogenetic tool employed to evaluate chromosomal damage subsequent to pesticide exposure. OBJECTIVES: To evaluate the pooled levels of total micronuclei (MN) and binucleated cells with micronuclei (MNC) in 1000 binucleated lymphocytes among population occupationally exposed to pesticides and further determine the more sensitive biomarker of CBMN. MATERIALS AND METHODS: A meta-analysis on the pooled levels of MN and MNC in binucleated lymphocytes among occupationally pesticide-exposed populations was conducted using STATA 10.0 software and Review Manager 5.0.24 in this study. RESULTS: We found significant differences in frequencies of MN and MNC in 1000 binucleated lymphocytes between pesticide-exposed groups and controls, and the summary estimates of weighted mean difference were 6.82 [95% confidence interval (95% CI): 4.86-8.78] and 5.08 (95% CI: 2.93-7.23), respectively. However, when we conducted sensitivity analyses further, only the MN remained statistically different, but not the MNC, the summary estimates of weight mean difference were 2.86 (95% CI: 2.51-3.21) and 0.50 (95% CI: -0.16-1.17), respectively. We also observed pesticide-exposed subjects had significantly higher MN frequencies than controls among smokers and nonsmokers, male and female populations, and American, Asian and European countries in stratified analyses. CONCLUSIONS: The frequency of MN in peripheral blood lymphocytes might be a more sensitive indicator of early genetic effects than MNC using the CBMN assay for occupationally pesticide- exposed populations.
Authors: Tatiana Vieira Souza Chaves; Muhammad Torequl Islam; Manoel Odoríco de Moraes; Marcus Vinícius Oliveira Barros de Alencar; Débora Cássia Vieira Gomes; Rodrigo Mendes de Carvalho; Sharbel Weider Maluf; Fabrício Pires de Moura do Amaral; Márcia Fernanda Correia Jardim Paz; Gilberto Santos Cerqueira; Hercília Maria Lins Rolim; João Marcelo de Castro E Sousa; Ana Amélia de Carvalho Melo-Cavalcante; Maria Elisabete Amaral de Moraes Journal: Environ Sci Pollut Res Int Date: 2017-05-16 Impact factor: 4.223
Authors: Maria Del Carmen Xotlanihua-Gervacio; Mirna Citlali Guerrero-Flores; José Francisco Herrera-Moreno; Irma Martha Medina-Díaz; Yael Yvette Bernal-Hernández; Briscia Socorro Barrón-Vivanco; Monserrat Sordo; Aurora Elizabeth Rojas-García Journal: Environ Sci Pollut Res Int Date: 2018-09-11 Impact factor: 4.223