S Sim1, A Chevrier2, M Garon3, E Quenneville4, A Yaroshinsky5, C D Hoemann6, M D Buschmann7. 1. Department of Chemical Engineering and Institute of Biomedical Engineering, Ecole Polytechnique de Montreal, P.O. Box 6079, Station Centre-Ville, Montreal, Quebec H3C 3A7, Canada; Biomomentum Inc., 970 Michelin St., Suite 200, Laval, Quebec H7L 5C1, Canada. Electronic address: sotcheadt.sim@polymtl.ca. 2. Department of Chemical Engineering and Institute of Biomedical Engineering, Ecole Polytechnique de Montreal, P.O. Box 6079, Station Centre-Ville, Montreal, Quebec H3C 3A7, Canada. Electronic address: anik.chevrier@polymtl.ca. 3. Biomomentum Inc., 970 Michelin St., Suite 200, Laval, Quebec H7L 5C1, Canada. Electronic address: garon@biomomentum.com. 4. Biomomentum Inc., 970 Michelin St., Suite 200, Laval, Quebec H7L 5C1, Canada. Electronic address: quenneville@biomomentum.com. 5. Vital Systems, Inc., 3701 Algonquin Rd, Suite 310 Rolling Meadows, IL 60008, USA. Electronic address: ayaroshinsky@gmail.com. 6. Department of Chemical Engineering and Institute of Biomedical Engineering, Ecole Polytechnique de Montreal, P.O. Box 6079, Station Centre-Ville, Montreal, Quebec H3C 3A7, Canada; Groupe de Recherche en Sciences et Technologies Biomédicales, Ecole Polytechnique de Montreal, P.O. Box 6079, Station Centre-Ville, Montreal, Quebec H3C 3A7, Canada. Electronic address: caroline.hoemann@polymtl.ca. 7. Department of Chemical Engineering and Institute of Biomedical Engineering, Ecole Polytechnique de Montreal, P.O. Box 6079, Station Centre-Ville, Montreal, Quebec H3C 3A7, Canada; Groupe de Recherche en Sciences et Technologies Biomédicales, Ecole Polytechnique de Montreal, P.O. Box 6079, Station Centre-Ville, Montreal, Quebec H3C 3A7, Canada. Electronic address: michael.buschmann@polymtl.ca.
Abstract
OBJECTIVE: The hand-held Arthro-BST™ device is used to map electromechanical properties of articular cartilage. The purpose of the study was to evaluate correlation of electromechanical properties with histological, biochemical and biomechanical properties of cartilage. METHOD: Electromechanical properties (quantitative parameter (QP)) of eight human distal femurs were mapped manually ex vivo using the Arthro-BST (1 measure/site, 5 s/measure, 3209 sites). Osteochondral cores were then harvested from different areas on the femurs and assessed with the Mankin histological score. Prior to histoprocessing, cores were tested in unconfined compression. A subset of the cores was analyzed with polarized light microscopy (PLM) to assess collagen structure. Biochemical assays were done on additional cores to obtain water content and glycosaminoglycan (GAG) content. The QP corresponding to each core was calculated by averaging all QPs collected within 6 mm of the core center. RESULTS: The electromechanical QP correlated strongly with both the Mankin score and the PLM score (r = 0.73, P < 0.0001 and r = -0.70, P < 0.0001 respectively) thus accurately reflecting tissue quality and collagen architecture. Electromechanical QP also correlated strongly with biomechanical properties including fibril modulus (r = -0.76, P < 0.0001), matrix modulus (r = -0.69, P < 0.0001), and log of permeability (r = 0.72, P < 0.0001). The QP correlated weakly with GAG per wet weight and with water content (r = -0.50, P < 0.0003 and r = 0.39, P < 0.006 respectively). CONCLUSION: Non-destructive electromechanical QP measurements correlate strongly with histological scores and biomechanical parameters providing a rapid and reliable assessment of articular cartilage quality.
OBJECTIVE: The hand-held Arthro-BST™ device is used to map electromechanical properties of articular cartilage. The purpose of the study was to evaluate correlation of electromechanical properties with histological, biochemical and biomechanical properties of cartilage. METHOD: Electromechanical properties (quantitative parameter (QP)) of eight human distal femurs were mapped manually ex vivo using the Arthro-BST (1 measure/site, 5 s/measure, 3209 sites). Osteochondral cores were then harvested from different areas on the femurs and assessed with the Mankin histological score. Prior to histoprocessing, cores were tested in unconfined compression. A subset of the cores was analyzed with polarized light microscopy (PLM) to assess collagen structure. Biochemical assays were done on additional cores to obtain water content and glycosaminoglycan (GAG) content. The QP corresponding to each core was calculated by averaging all QPs collected within 6 mm of the core center. RESULTS: The electromechanical QP correlated strongly with both the Mankin score and the PLM score (r = 0.73, P < 0.0001 and r = -0.70, P < 0.0001 respectively) thus accurately reflecting tissue quality and collagen architecture. Electromechanical QP also correlated strongly with biomechanical properties including fibril modulus (r = -0.76, P < 0.0001), matrix modulus (r = -0.69, P < 0.0001), and log of permeability (r = 0.72, P < 0.0001). The QP correlated weakly with GAG per wet weight and with water content (r = -0.50, P < 0.0003 and r = 0.39, P < 0.006 respectively). CONCLUSION: Non-destructive electromechanical QP measurements correlate strongly with histological scores and biomechanical parameters providing a rapid and reliable assessment of articular cartilage quality.
Authors: Lina Acevedo; Lukas Iselin; Majoska H M Berkelaar; Gian M Salzmann; Francine Wolf; Sandra Feliciano; Nicole Vogel; Geert Pagenstert; Ivan Martin; Karoliina Pelttari; Andrea Barbero; Markus P Arnold Journal: Cartilage Date: 2020-09-22 Impact factor: 3.117
Authors: Jan C Schagemann; Nicola Rudert; Michelle E Taylor; Sotcheadt Sim; Eric Quenneville; Martin Garon; Mathias Klinger; Michael D Buschmann; Hagen Mittelstaedt Journal: Cartilage Date: 2016-01-22 Impact factor: 4.634
Authors: Adele Changoor; Martin Garon; Eric Quenneville; Shelley B Bull; Karen Gordon; Pierre Savard; Michael D Buschmann; Mark B Hurtig Journal: Cartilage Date: 2020-06-05 Impact factor: 3.117