| Literature DB >> 25168178 |
Christian Sibbersen1, Lennart Lykke, Niels Gregersen, Karl Anker Jørgensen, Mogens Johannsen.
Abstract
The direct conjugation of a labeled proteome to a cleavable azide resin utilizing the copper-catalyzed azide alkyne cycloaddition is demonstrated. The procedure omits the classical streptavidin- and biotin-based affinity enrichment step and represents an operationally simpler, cheaper and less contaminated alternative for protein purification.Entities:
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Year: 2014 PMID： 25168178 DOI： 10.1039/c4cc05246c
Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222